CSBR: Living Stock Collections: A "Biological Gold Mine" of plant natural products and pathways for biochemical and evolutionary studies

马萨诸塞大学阿默斯特分校（University of Massachusetts Amherst）获得了一个奖项，以改进一个大型植物细胞培养图书馆（PCCL），该图书馆最近由工业界捐赠给该大学，并使其可供研究界使用。PCCL包括2200多种独特的植物物种，是世界上最大和最多样化的植物细胞培养集合（约占植物种类的69%），也是西半球唯一的此类集合。PCCL是新型天然产物及其生物合成所需遗传信息的独特而有价值的来源。重要的是，PCCL对学术研究界和工业界的可用性具有广泛的社会影响潜力，通过发现和开发新的化合物，应用于生态可持续性（绿色化学，可再生资源），农业（用新型抗微生物或抗真菌剂预防作物损失，增强食品和饲料作物的营养）以及人类健康和保健。此外，PCCL为本科教育和推广提供了宝贵的资源，作为让学生参与植物巨大的遗传和生化多样性的材料来源。具体而言，该项目将简化细胞培养维护方案，优化快速培养规模和传播到研究界的程序。PCCL数据库将现代化，为研究界部署一个网络界面，并将开发一个维持和分发PCCL材料的可持续模式。该方法将改善冷藏条件的发展，通过减缓生长以增加转移之间的时间，减少维持活培养物的劳动力和成本。低温保存方法将进行测试，以优化尽可能多的活培养物转移到低温保存状态。冷冻保存的材料将被复制，存放在美国农业部设在柯林斯堡的国家遗传资源保存中心。将对培养基进行评估，以减少维持培养基所需的培养基数量。最后，一个更新的PCCL分类法将集成到一个基于web的界面，以提高PCCL的可访问性（pc.library.umass.edu）。

An award is made to the University of Massachusetts Amherst to improve a large Plant Cell Culture Library (PCCL) that was recently donated to the university from industry, and make it available to the research community. The PCCL includes over 2200 unique plant species, the largest and most diverse collection of plant cell cultures in the world (representing approximately 69% of plant orders) and is the only such collection in the western hemisphere. The PCCL is a unique and valuable source of novel natural products and the genetic information required for their biosynthesis. Significantly, the availability of the PCCL to the academic research community and to industry has the potential for broad societal impact through the discovery and development of new compounds with applications in ecological sustainability (green chemistry, renewable resources), agriculture (prevention of crop loss with novel anti-microbial or anti-fungal agents, nutritional enhancement of food and feed crops) and human health and wellness. Additionally, the PCCL provides a valuable resource for undergraduate education and outreach as a source of material with which to engage students in the vast genetic and biochemical diversity of plants. Specifically, this project will streamline cell culture maintenance protocols and optimize procedures for rapid culture scale-up and dissemination to the research community. The PCCL database will be modernized, a web interface for the research community deployed, and a sustainable model for maintaining and distributing PCCL materials will be developed. The approach will improve the development of cold storage conditions that will reduce the labor and cost of maintaining the living cultures by slowing growth to increase the time between transfers. Cryopreservation methods will be tested to optimize the transfer of as many living cultures as possible to a cryopreserved state. Cryopreserved materials will be duplicated to be deposited in the National Center for Genetic Resources Preservation, a USDA facility in Fort Collins. Culture media will be evaluated to reduce the amount of media required for culture maintenance. Finally, an updated PCCL taxonomy will be integrated into a web-based interface to improve accessibility of the PCCL (pcc.library.umass.edu).