MRI: Acquisition of a High Resolution Mass Spectrometer/UPLC System

密苏里大学获得了一个高分辨率质谱仪和一个超高性能液相色谱系统。该仪器将用于帮助我们进一步了解包括大豆、玉米和接骨木在内的各种植物中的蛋白质。它还将用于在蛋白质水平上检查动物的生育力损失，以支持农业。该大学正在与埃文斯分析集团（EAG）哥伦比亚公司（一家位于密苏里州哥伦比亚市的符合GLP和cgmp标准的产品开发和分析测试服务公司）合作，为本科生提供持续的行业实习计划。每年春季本科实习生都要参加为期四周的强化培训课程。这门深入的课程为高素质的本科生提供了一个发展分析技能的机会，重点是分离和质谱，为EAG哥伦比亚大学的暑期实习生做准备。生物化学和化学本科学生也将在高级实验课上使用该仪器，以进一步了解现代仪器。质谱法是测定蛋白质等大分子结构的重要方法。这种方法也可以用于检测蛋白质的修饰。获得高分辨率质谱仪将使该项目的研究人员能够进行定量蛋白质分析，开发新的分析能力，并改善密苏里大学的仪器使用。该仪器的主要用户有活跃的研究计划，检查各种植物和动物的蛋白质变化。

An award is made to the University of Missouri to acquire a high resolution mass spectrometer coupled with an ultra-high performance liquid chromatography system. The instrument will be used to help further our understanding of proteins in a variety of plants including soybean, corn, and elderberry. It will also be used to examine fertility loss in animals at the protein level in support of agriculture. The University is collaborating with Evans Analytical Group (EAG) Columbia (a GLP and CGMP-compliant product development and analytical testing services company based in Columbia, MO) in a continuing industry internship program for undergraduates. Each spring undergraduate interns participate in an intensive four-week training course. This in-depth course offers a select set of high-caliber undergraduates an opportunity to develop analytical skills, with an emphasis on separations and mass spectrometry, in preparation for being employed by EAG Columbia as summer interns. Biochemistry and Chemistry undergraduate students will also use the instrument in advanced lab classes to further their understanding of modern instrumentation.Mass spectrometry is an essential method for determining the structure of large biomolecules such as proteins. It is also possible to use this method to examine modifications to proteins. The acquisition of the high resolution mass spectrometer will allow the investigators of the project to perform quantitative protein analyses, develop new analysis capabilities, and improve instrument access at the University of Missouri. The major users of the instrument have active research programs examining protein changes in a variety of plants and animals.