Profiling tissue protein, elemental ions and nanoparticle distributions

该项目旨在研究蛋白质-蛋白质之间的相互作用，这些相互作用对于在不断变化的组织环境中的稳态细胞信号和生存能力至关重要。中心目标是开发和验证结合尖端组织成像模式的方案，以绘制和表征天然和修饰蛋白质、元素离子和包括纳米颗粒和纳米载体在内的药理制剂的组织分布。其目的是将新的组织扫描质谱学技术与基于X射线的微探针光谱和先进的多参数细胞术相结合，以确定蛋白质、离子和药物在组织中的空间分布。这种方法可能提供关于维持体内平衡控制以及药物或纳米粒在生物组织中的含量、分布和潜在代谢的新信息。

This project aims to investigate protein-protein interactions that are crucial to homeostatic cell signalling and viability in a changing tissue environment. The central goal is to develop and validate protocols to combine cutting-edge tissue imaging modalities to map and characterise tissue distributions of native and modified proteins, elemental ions and pharmacological agents including nanoparticles and nanovehicles. The aim is to use novel tissue scanning mass spectrometry techniques in conjunction with X-ray-based microprobe spectroscopy and advanced multi-parameter cytometry to identify spatial distributions of proteins, ions and drugs in tissues. This approach may provide new information about the maintenance of homeostatic control and the content, distribution and potential metabolism of drugs or nanoparticles within biological tissues.