MRI: Acquisition of a Leica SP8 WLL SuperResolution and Light Sheet Microscope

纽约城市大学亨特学院（CUNY）获得了一个奖项，赞助购买一种具有超分辨率和数字光片功能的新型激光扫描共聚焦显微镜（lms - dls），该显微镜将被安置在开放访问的多用户生物成像核心设施中。所要求的显微镜将帮助来自多个部门的教师不断增长的研究和教学需求，以及亨特学院，其他城市大学校园和其他地区机构的下一代科学家的培训需求。高中、本科和研究生将受益于创新的、前沿的荧光成像技术的实践培训，通过课程作业和研究，这将加强他们的教育和指导，对他们未来在生物科学领域的职业生涯至关重要。亨特学院一直致力于通过提供卓越和活跃的研究环境，为未被充分代表的少数民族研究人员提供追求科学事业的机会。已经到位的学院赞助项目鼓励更广泛地参与科学研究，这将通过购买最先进的显微镜系统得到加强。新的LSM-DLS不仅可以满足现有教师的需求，还可以满足学院和大学内几个部门的未来需求。它将改变和推进收集定量、高空间和时间分辨率图像的研究能力，以回答细胞生物学、发育生物学、神经科学和生物材料纳米制造中的关键问题。它将支持NSF和其他资助的广泛领域的研究，包括：(a)转录开关，(b)亚细胞分子定位，(c)细胞骨架组织，(d)外显体功能，(e)髓磷脂形成，以及(f)脑中的树突棘形态动力学。该技术可以实现大范围样品的可视化，并以高分辨率实时检测从单分子到整个生物体的发育。为了完成这些项目，研究人员将利用LSM-DLS设备的全部功能，包括3D重建、实时成像、光谱分解和光片照明，为他们提供前所未有的能力，在亚衍射尺度上可视化从无脊椎动物卵母细胞到脊椎动物大脑的结构。这些研究的结果将发表在同行评议的科学期刊上，在科学会议上提出，并用于教学和公共宣传活动。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

An award is made to Hunter College of the City University of New York (CUNY) to sponsor the acquisition of a new laser scanning confocal microscope with super-resolution and digital light sheet capabilities (LSM-DLS), which will be housed in the open-access, multi-user Bio-Imaging Core Facility. The requested microscope will assist the growing research and teaching needs of faculty from multiple departments, as well as the training needs of the next generation of scientists across several disciplines at Hunter College, other CUNY campuses, and at other regional institutions. High school, undergraduate and graduate students will benefit from hands- on training in innovative, leading-edge fluorescence imaging techniques through course work and research, which will enhance their education and mentorship important for their future careers in biological sciences. Hunter College has continued commitment to increase the opportunities for under-represented minority researchers to pursue scientific careers by providing an environment of excellence and active research. Already in-place College sponsored programs encourage broader participation in scientific research that will be strengthen by the acquisition of this state-of-the-art microscope system.The new LSM-DLS will not only meet the needs of current faculty, but will also meet future needs of several departments within both the College and University. It will transform and advance the research capabilities for collecting quantitative, highly spatial and temporal resolution images in order to answer critical questions in cell biology, developmental biology, neuroscience, and nanofabrication of biomaterials. It will support NSF- and other funded research in a broad range of areas including: (a) transcriptional switches, (b) sub-cellular molecular localization, (c) cytoskeleton organization, (d) exosome function, (e) myelin formation, and (f) dendritic spine morphology dynamics in the brain. This technology allows for the visualization of a wide range of samples, and detection from single molecules to whole organism development in real time at high resolution. To perform these projects, investigators will utilize the full range of capabilities of the LSM-DLS setup, including 3D reconstruction, live imaging, spectral unmixing and light-sheet illumination, providing them with unprecedented abilities to visualize structures at a sub-diffraction scale from invertebrate oocytes to the vertebrate brain. The results of these studies will be published in scientific peer-reviewed journals, presented at scientific meetings and used in both teaching and public outreach activities.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.