MRI: Acquisition of a Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometer to Enhance Research and Education

该奖项由主要研究仪器和化学研究仪器项目支持。俄亥俄大学正在获得一个矩阵辅助激光解吸/电离、飞行时间、质谱仪（MALDI-TOF-MS），配备实时直接分析（DART）源，以支持Stephen Bergmeier教授和他的同事Eric Masson、Justin Holub、Amir Farnoud和Jessica White。一般来说，质谱（MS）是用于鉴定和表征复杂样品中少量化学物质的关键分析方法之一。MALDI TOF结合了温和的电离（非常适合产生肽，蛋白质，核酸，碳水化合物，合成聚合物和其他类似大小的物种的完整离子）与检测模式，在广泛的质量范围内提供了灵敏度和准确性之间的良好平衡。这种高度敏感的技术可以识别和确定分子的结构。此次收购加强了该大学和地区的研究基础设施。该仪器通过让不同的学生使用这种现代分析技术来扩大参与。MALDI-TOF-MS也被用于涉及来自俄亥俄州阿巴拉契亚地区的学生和俄亥俄州东南部一些文理学院的教师的外展活动，包括：玛丽埃塔学院、肖尼州立大学和里约热内卢格兰德大学。颁发质谱仪的目的是为了加强各级的研究和教育。它特别影响合成的有机和药用化合物的表征。该仪器用于开发以肽为基础的配体，调节细胞中治疗相关的信号转导途径。此外，它还有利于利用curcubit[n]uril家族的大环探索超分子化学，以及利用无机化合物作为光化学疗法及其与DNA的相互作用。质谱计用于了解纳米材料破坏质膜的机制，并用于测量和成像固体表面上的化学物质。MALDI光谱仪还用于研究具有潜在抗生素用途的酚溶性调节素，以及筛选合成或天然聚合物、金属配合物、细菌菌株、复杂碳水化合物和合成无机化合物。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. Ohio University is acquiring a matrix-assisted laser desorption/ionization, time-of-flight, mass spectrometer (MALDI-TOF-MS) equipped with direct analysis in real time (DART) sources to support Professor Stephen Bergmeier and colleagues Eric Masson, Justin Holub, Amir Farnoud and Jessica White. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. MALDI TOF combines gentle ionization (ideal for producing intact ions of peptides, proteins, nucleic acids, carbohydrates, synthetic polymers, and other similarly sized species) with a detection mode that offers an excellent balance between sensitivity and accuracy across a wide mass range. This highly sensitive technique allows identification and determination of the structure of molecules. The acquisition strengthens the research infrastructure at the university and region. The instrument broadens participation by involving diverse students with this modern analytical technique. The MALDI-TOF-MS is also used in outreach activities involving students from Appalachian Ohio and faculty from a number of liberal arts colleges in southeast Ohio including: Marietta College, Shawnee State University and the University of Rio Grande.The award of the mass spectrometer is aimed at enhancing research and education at all levels. It especially impacts the characterization of synthesized organic and medicinal compounds. The instrumentation is used for developing peptide-based ligands that modulate therapeutically-relevant signal transduction pathways in cells. In addition, it benefits the exploration of supramolecular chemistry using macrocycles of the curcubit[n]uril family and the use of inorganic compounds as photochemotherapeutics and their interactions with DNA. The mass spectrometer is used to understand mechanisms through which nanomaterials disrupt the plasma membrane and for measuring and imaging chemicals on solid surfaces. The MALDI spectrometer is also employed in studies of phenol-soluble modulins which have potential uses as antibiotics, and in screening synthetic or natural polymers, metal complexes, bacterial strains, complex carbohydrates and synthetic inorganic compounds.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.