Control of DNA translocation through solid-state nanopores with integrated electrodes

我们解决了短DNA片段(或纳米颗粒等)的移位问题。在外加电场的影响下，通过薄固态膜中的人造纳米孔。本文的总体目标是根据施加的电压从可观察到的电流信号中得出关于移位DNA的详细结构和动力学性质的结论，最理想的是直到碱基对序列。我们的具体目标是探索通过一个或几个直接集成到膜上的电极来控制和检测DNA转位的新概念。主要的重点将是对作用在DNA上的电动和流体动力以及通过各种电极的电流进行建模和优化。几个定量未知的模型参数将通过基本实验来确定。

We address the translocation of short DNA fragments (or nanoparticles etc.) through an artificial nanopore in a thin solid-state membrane under the influence of an externally applied electrical field. The general objective in this context is to draw conclusions from the observable current signals in response to the applied voltage with respect to the detailed structural and dynamical properties of the translocating DNA, ideally up to the sequence of the base pairs. Our specific goal is to explore new concepts of controlling and detecting DNA translocation by means of one or several electrodes which are integrated directly into the membrane. The main focus will be on modeling and optimization of the electrokinetic and hydrodynamic forces acting upon the DNA and of the electric currents through the various electrodes. Several quantitatively unknown model parameters will be determined by means of a basic experiment.