Integrated OMICS Study of the Beneficial Effects of Egg Shell Membrane-derived Resistant Protein on Intestinal Bacteria

据报道，摄入抗性蛋白(RP：不可消化的蛋白)可以发挥有益的作用。然而，肠道微生物区系参与这种有益的影响还没有完全阐明。以前的研究表明，蛋壳膜(ESM)和清酒蛋糕(Profiel：PFB)含有大量的RP。将48只C57BL6J雄性小鼠随机分为以下6组：(1)对照组(Ctrl)，(2)高脂饮食(HFD)，(3)高脂饮食(Hesm)，(4)高脂饮食+8%ESM+抗生素混合物(ESMAbx)，(5)高脂饮食+30%PFB(HPFB)，(6)HFD+30%PFB+抗生素混合物(PFBAbx)。与Ctrl组相比，喂食HFD的小鼠显示出体重、内脏脂肪质量、肝脏重量、肝脏甘油三酯和肝脏胆固醇增加。与HFD组相比，Hesm组和ESMAbx组小鼠体重增加均明显减少。然而，与HFD组相比，HPFB和PFBAbx组小鼠的体重增加没有受到影响。口服葡萄糖耐量试验在这些治疗组中没有受到影响。PFB治疗可降低血清和肝脏胆固醇水平。与Ctrl组相比，HFD组大鼠结肠内容物中免疫球蛋白(IgA)水平升高。与HFD组相比，ESM治疗对结肠内容物中IgA水平无明显影响。经PFB处理后，结肠内容物中的IgA水平略有升高。

Consumption of resistant proteins (RP: non-digestible proteins) has been reported to exert beneficial functions. However, the involvement of gut microbiota in such beneficial effects is not fully elucidated. Previous studies have shown eggshel membrane (ESM) and sake cake (Profiber: PFB) contain a large amount of RP. Here, I performed the first animal experiment to investigate the effects of dietary ESM or PFB on metabolic dysfunctions and gut dysbiosis induced by high-fat diet (HFD).A total of 48 C57BL6J male mice was assigned randomly to the following six dietary groups: (1) Control diet (Ctrl), (2) high-fat diet (HFD), (3) HFD with 8% ESM (HESM), (4) HFD with 8% ESM+ antibiotics mix (ESMAbx), (5) HFD with 30% PFB (HPFB), (6) HFD with 30% PFB + antibiotics mix (PFBAbx). Compared with Ctrl group, mice fed an HFD showed increased body weight, visceral fat mass, liver weight, liver triglycerides, and liver cholesterol. Compared with the HFD group, the mice body weight gains were reduced in both of HESM and ESMAbx groups. However, the mice body weight gains were not affected in HPFB and PFBAbx groups compared that in the HFD group. The oral glucose tolerance test was not affected in these treatment groups. PFB treatment reduced the level of cholesterol in serum and liver. Compared with the Ctrl group, HFD showed increased level of immunoglobulin (IgA) in colon content. ESM treatment did not affect IgA level in colon content compared with that of HFD group. PFB treatment was shown to slightly increase the IgA level in colon content.