Marburg core facility for Interaction, Dynamics and Assembly of biomolecular Structures

深入分析生物分子复合物的动力学和结构特征及其分子机制对于现代生命科学至关重要，也是理解疾病和生物工程的关键。氢氘交换质谱（HDX）和高通量结晶平台（HTP-X）支持的X射线晶体学是高度互补的最先进的高分辨率动态和结构分析方法。随着2015年在德国一所大学安装了迄今为止第一个也是唯一一个自动化HDX管道，我们经历了科学界对核心设施的日益增长的需求（如支持信函所证明的那样），这两种技术将在科学和行政支持的标准化设置中相互关联。因此，我们建议DFG核心设施马尔堡核心设施的相互作用，动力学和生物分子组装结构（MIDAS）集成在多学科和高度可见的合成微生物学中心（SYNMIKRO）的菲利普斯大学。MIDAS将其创新的自动化HDX管道与已建立的HTP-X合并为一个独特的科学基础设施，可供广泛的国家和国际用户社区使用。在三年的供资期内，MIDAS将成为一个高度专业化和标准化的合作框架，并具有明确的治理结构。这将通过在线用户手册中记录的用户界面、数据管理系统和预定义的工作包（WP）实现，并由细则（Nutzungsordnung）进行管理。将通过测试用户熟练度（TUF）持续监测CF（包括其WP）的性能。此外，MIDAS将通过其年度用户会议和2018年HDX和结构方法的国际暑期学校促进沟通和教育。总的来说，随着拟议的技术，科学和行政支持的安装，MIDAS将成为一个高度可见的DFG核心设施;建模如何将最先进的技术经济地合并到优秀的功能单元中，作为合作研究计划，中心和卓越集群中的核心单元。

In-depth analysis of the dynamic and structural features of biomolecular complexes and their molecular mechanisms is crucial for the modern life sciences, but also understanding disease and bioengineering. Hydrogen-Deuterium eXchange mass spectrometry (HDX) and X-ray crystallography empowered by high-throughput crystallization platforms (HTP-X) are highly complementary, state-of-the-art methods for the dynamic and structural analysis at high resolution. With the installation of the up to now first and only automated HDX pipeline at a German University in 2015, we experienced an increasing need of the scientific community for a core facility (as evidenced by the Support letters), where both technologies would be interlinked in a standardized setup with scientific and administrative support. Therefore, we propose the DFG core facility Marburg core facility for Interactions, Dynamics and biomolecular Assembly Structure (MIDAS) integrated in the multidisciplinary and highly visible LOEWE Center of Synthetic Microbiology (SYNMIKRO) of the Philipps-University. MIDAS will merge its innovative automated HDX pipeline with established HTP-X into a unique scientific infrastructure available to a broad national and international user community. In the 3-year funding period, MIDAS will become a highly professionalized and standardized CF with a defined governance structure. This will be enabled by a user interface, data management system and predefined work packages (WPs) documented in an Online User Manual and regulated by bylaw (Nutzungsordnung). Performance of the CF including its WPs will be continuously monitored through Testing User Friendlyness (TUF). Further, MIDAS will promote communication and education through its annual user meetings and an international summerschool on HDX and structural methods in 2018. Taken together, with the installation of the proposed technical, scientific and administrative support, MIDAS will become a highly visible DFG core facility; modeling how the state-of-the-art technology can be economically merged into excellent functional units serving as core units within collaborative research initiatives, centers and excellence clusters.