Quantitative Interactomics to characterize lncRNPs in vitro and in vivo

lncRNA的功能目前在包括神经元在内的任何系统中都不完全清楚。在此，我们建议使用我们开发的定量rna为中心的相互作用组学，将蛋白质结合物映射到这个优先计划中的不同神经元lncRNA。此外，我们的目标是进一步将其发展为一种敏感的体内技术，该技术将允许从神经元细胞中纯化lncRNPs，以增强lncRNA功能的表征。该方法可扩展到其他RNA物种，如miRNA。

The function of lncRNA is currently incompletely understood in any system, including neurons. We here propose to use our developed quantitative RNA-centered interactomics to map protein binders to diverse neuronal lncRNA within this Priority Program. Additionally, we aim to further develop this to a sensitive in vivo technique that will allow purification of lncRNPs from neuronal cells to enhance the characterization of lncRNA functionality. This approach is extendable to other RNA species such as miRNA.