Proteomic analysis of the chnages in blood induced by therapeutic hypothermia

心脏骤停后治疗性低温的应用越来越多，但其多效性作用尚未完全阐明。为了分析这些机制，我们对比研究了9名接受治疗性低温的患者在低温诱导和复温后引起的血浆蛋白质组变化。我们利用iTRAQ标记串联质谱仪来评估蛋白质的定量变化。这项分析总共鉴定了188种蛋白质。生物信息学分析表明，在低温条件下，丝氨酸蛋白酶抑制剂和防御/免疫蛋白的表达显著上调。复温后，载脂蛋白和补体成分蛋白的表达也显著上调。在9名患者中，3名患者已经康复，没有任何神经并发症，但另外6名患者存在严重的神经缺陷。这些结果与几种属于氧化应激和先天免疫的蛋白质水平有关。

Therapeutic hypothermia after cardiac arrest is increasingly utilized, but its pleiotropic effects are as yet incompletely elucidated. In order to analyze these mechanisms, we comparatively investigated plasma proteomic changes induced by both induction of hypothermia and rewarming in nine patients undergoing therapeutic hypothermia. We utilized iTRAQ labeling tandem mass spectrometry to assess quantitative protein changes. In total, the analysis identified 188 proteins. Bioinformatic analyses showed significant up-regulation of serine protease inhibitor and defense/immunity protein at hypothermia. After rewarming, both apolipoprotein and complement component proteins were also significantly up-regulated. Out of nine patients, three patients have recovered without any neurological complications, but the other six patients have had serious neurological defects. These outcomes were associated with the levels of several proteins belonging to oxidative stress and innate immunity.