Functional modification of live cells by focused ion beam doping

提出了一种利用聚焦离子束(FIB)对活细胞进行重金属注入的新方法。我们通过FIB注入的方法将Au和As离子掺杂到活细胞中，然后评估生物能量储存分子三磷酸腺苷(ATP)分子在细胞内的水平。与未植入对照细胞相比，植入细胞的三磷酸腺苷水平发生了改变。我们的离子注入技术可能比传统方法更准确地定量解释掺杂剂的剂量依赖效应。

A novel heavy-metal implantation method for living cells using focused ion-beam (FIB) were proposed. We performed Au and As ion doping into living cells by using the FIB implantation method; then intracellular level of adenosine triphosphate (ATP) molecule, which is the energy storing molecule for organisms, was evaluated. The ATP level of the implanted cells was found to be modified compared with that of the non-implanted control cells. Our ion implantation technique may be a more accurate tool to quantitatively elucidate the dose-dependent effects of dopants than the conventional methods.