A new culture method using bacteria-adhesive particles

采用分子方法研究了好氧和酸性条件下粪便细菌培养前后3 d细菌组成的变化。在有氧条件下，部分肠道细菌能够在含有10%胎牛血清的汉克平衡盐溶液中生长。正如预期的那样，我们观察到兼性厌氧菌的显著增加和专性厌氧菌的减少。然而，厌氧细菌脆弱拟杆菌在需氧培养7天后可以重新生长。末端限制性酶切长度多态性分析表明，好氧培养后的峰值数量减少，表明细菌多样性丧失。在培养物中维持pH为4.5可抑制细菌生长，提示pH控制可能有助于预防细菌感染。

We investigated changes of bacterial composition before and after 3 days culture of fecal bacteria under the aerobic or acidic condition by the molecular methods. A part of intestinal bacteria was able to grow in Hank's balanced salt solution containing 10% fetal calf serum under the aerobic condition. As was expected we observed the significant increase of facultative anaerobes and decrease of obligate anaerobes. However, Bacteroides fragilis, an anaerobe, could regrow after 7 days aerobic culture. Terminal restriction length polymorphism analysis demonstrated the decreased number of peaks after the aerobic culture, suggesting loss of bacterial diversity. The maintenance at pH 4.5 in cultures resulted in the inhibition of bacterial growth, suggesting possible contribution of pH control to prevention of bacterial infection.