Cancer stem cell for peritoneal metastasis in abdominal cavity

结果发现，一小部分CD 90 + CD 45-的人腹腔游离细胞具有间充质干细胞的特性，在TGF β刺激下可分化为肌成纤维细胞，腹腔注射MKN 45可显著提高裸鼠腹膜转移的形成率。组织学检查发现，许多MSC在转移结节中植入，主要位于纤维区。酪氨酸激酶抑制剂达沙替尼在体外强烈抑制MSC的增殖，但不抑制MKN 45。然而，口服达沙替尼显著抑制了MKN 45腹膜转移的发展，并导致转移性结节的纤维形成减少。流式细胞术检测CD 326+肿瘤细胞率可作为判断腹膜转移严重程度和化疗有效性的可靠诊断生物标志物。

We found that a minor subset of human intraperitoneal free cells with CD90+CD45- displayed the characteristics of mesenchymal stem cell, and differentiated into myofibroblast by the stimulation with TGFb, and intraperitoneal coinjection of with MKN45 significantly enhanced the rate of metastatic formation in the peritoneum of nude mice. Histological examination revealed that many MSC were engrafted in metastatic nodules and were mainly located at the fibrous area. Dasatinib, a tyrosine kinase inhibitor, strongly inhibited the proliferation of MSC but not MKN45 in vitro. Nevertheless, oral administration of Dasatinib significantly inhibited the development of peritoneal metastasis of MKN45, and resulted in reduced fibrillar formation of metastatic nodules. The rate of CD326+ tumor cells measured with FACS could be a reliable diagnostic biomarker to determine the severity of peritoneal metastasis as well as effectiveness of chemotherapy.