Reconstruction of the normal vasculature for the strategy of new cancer therapy.

我们假设，肿瘤中正常血管的重建可能会导致低氧条件的改善，有助于潜在的抗癌药物输送的改善。将肿瘤细胞注射到SCID小鼠背侧皮下。2周后，给荷瘤小鼠腹腔注射顺铂，以消退肿瘤和肿瘤血管为第一步。两周后，将人内皮细胞或人胚胎来源的内皮细胞移植到肿瘤的坏死区，以诱导第二步正常的血管生成。显微镜观察显示，移植的内皮细胞与宿主小鼠的血管形成了吻合。内皮细胞重建的血管显示肿瘤血管的正常化，导致肿瘤体积显著缩小。因此，通过构建可靠的药物输送系统和改善低氧条件，该模型是未来开发新的癌症治疗方法的有用模型。

We hypothesized that reconstruction of normal blood vessels in tumors may lead to improvement of hypoxic conditions, contributing to potential improvements in the delivery of anticancer drugs. Tumor cells were injected into the dorsal subcutis of SCID mice. After 2 weeks, the tumor-bearing mice were injected cisplatin intraperitoneally to regress tumors and tumor vessels for the first step. Two weeks later, human ECs or human ES-derived ECs were transplanted into necrotic regions of the tumor to induce normal angiogenesis for the second step. Microscopic observations revealed that the transplanted ECs formed anastomoses with the host mouse vasculature. Blood vessels reconstituted by ECs exhibited the normalization of tumor vasculature, resulting in a significant reduction in the tumor volume. Thus, this model is a useful model for the future development of new cancer therapies by the construction of reliable drug delivery systems and the improvement of hypoxic conditions.