A simple sequencing system of complete chloroplast genome using long PCR and NGS in coniferous species

建立了针叶植物叶绿体基因组的两个简单的全序列测定系统。一种是使用下一代测序仪(NGS)的测序系统，以及利用属间高度保守区域的排列信息设计的长PCRS。另一个是利用从粗叶绿体部分提取的基因组DNA的下一代测序和生物信息学的系统。使用这两种测序系统中的任何一种都有可能完全破译许多针叶树种的叶绿体基因组。

Two simple complete-sequencing systems of chloroplast genome were constructed for Coniferophyta species. One was the sequencing system using a next generation sequencer (NGS), and long PCRs designed with the arrangement information of the highly conserved regions among genera. The other was the system using the next generation sequencing of the geneme DNA extracted from crude chloroplast fraction of the leaf and bioinformatics. It could be possible to decode the chloroplast genome completely in a lot of coniferous species by using either of these two sequencing systems.