Preparation of biomimetic prosthesis prepared from cultured smooth muscle cells and endothelial cells.

众所周知，动脉假体，特别是小直径的动脉假体，通常与血栓形成和增生有关。此外，必须保持机械强度以承受动脉压。已经进行了许多不成功的试验，使用由涤纶或膨胀聚四氟乙烯（e-PTFE）制成的人工假体接种内皮细胞以防止血栓形成和增生。为了解决这些问题，已经尝试了静脉同种异体移植物和Dardik移植物（用戊二醛处理的脐静脉）。最近，当胸廓内动脉的同种异体动脉移植物用于冠状动脉搭桥手术时，已经看到了改善的结果。鉴于此，我们认为动脉结构可能在防止动脉增生和抵抗动脉压方面起作用。在这些条件下，由弹性板层组成的动脉结构被保存，并且保持拉伸强度。胶原<51>蛋白、纤连蛋白和纤维蛋白原的存在增强了血管内皮细胞与假体表面的粘附。这些试剂还增强了^ Cr血小板的粘附<51>。然而，内皮细胞的预附着的假体阻止血小板adhesion.This研究表明，生产一种新的仿生动脉假体，应该证明是有用的，特别是在血栓形成和增生的机制的调查。这项工作的一部分，由日本教育，科学和文化部的赠款支持。

It is well know that arterial prostheses, especially those of small diameter, are often associated with thrombus formation and hyperplasia. In addition, it is necessary to retain the mechanical strength to withstand arterial pressure.A number of unsuccessful trials have been carried out using artificial prostheses made of Dacron or expanded polytetra fluoethylene (e-PTFE) seeded with endothelial cells to prevent thrombus formation and hyperplasia. To solve these problems, vein allograft and Dardik graft (umbilical vein treated with glutaraldehyde) have been tried. More recently, improved results have been seen when arterial allografts of the internal thoracic artery have been used in aortocoronary bypass operations. In view of this we considered that the arterial architecture may play a role in the prevention of hyperplasia and reistance to arterial pressure.In this study, a biomimetic prosthesis was prepared from canine arterial wall by extraction with formic acid. Under these conditions, the arterial architecture consisting of elastic lamella was conserved and the tensile strength was retained. Attachment of ^<51>Cr-endothelial cells to the surface of the prosthesis was enhanced in the presence of collagen, fibronectin, and fibrinogen. These agents also enhanced the adhesion of ^<51>Cr platelets. However, preattachment of endothelial cells to prostheses prevented platelet adhesion.This study demonstrates the production of a novel biomimetic arterial prosthesis that should prove useful, particularly in an investigation of the mechanism of thrombosis and hyperplasia.This work was supported in part by a grant from the Ministry of Education, Science and Culture of Japan.