Development of a novel method detecting interaction between proteins and DNAs

为了建立一种检测蛋白质与其结合DNA相互作用的灵敏方法，我们采用了基于实时PCR的方法，将探针DNA结合抗体与蛋白质结合，并将靶DNA结合另一个探针DNA。我们用该方法检测了蛋白质与目标dna之间的相互作用，但灵敏度远低于其他正宗方法。因此，这种新方法的实际应用还需要进一步的改进。

To develop a sensitive method detecting interaction of proteins with their binding DNAs, we applied a real-time PCR based methods with probe DNA-conjugated antibodies to a protein and also the target DNA conjugated another probe DNA. We detected interaction between a protein and the target DNAs by the method, but the sensitivity was much lower than that by the other authentic method. Thus, additional improvement is required for the practical use of this new method.