Establishment of ink jet technology to develop high throughput bio-assay system

利用喷墨打印技术和纸基材开发了一种新型的生物检测系统。喷墨打印具有在各种条件下对细菌和营养物质进行高精度计量分配的潜力。纸张有可能被用作容纳细菌和营养物质的容器，使用后很容易通过燃烧处理。滤纸首先浸入聚苯乙烯的甲苯溶液中，干燥后进行疏水处理。甲苯被印在滤纸的几个部分上，使它们再次亲水。亲水性部分印有可通过酸水解调节粘度的琼脂介质。将细菌手动分配到培养基上，观察到细菌菌落在培养基干之前至少生长24小时。具有聚合引发剂的聚乙烯醇也被成功地应用于介质。这一生物检测系统有望应用于各种细菌和酵母菌的孢子和酶。

A novel bioassay system using ink jet printing technology and a paper substrate has been developed. Ink jet printing has potential for high-precision metered dispensing of bacteria and nutrients in various conditions. Paper has the potential to be used as a container to accommodate bacteria and nutrients and is easily disposed of by combustion after use. Filter paper was first immersed in a toluene solution of polystyrene and hydrophobized after drying. Toluene was printed on a few sections of the filter paper to make them hydrophilic again. An agar medium with its viscosity adjusted by acid hydrolysis was printed on the hydrophilic sections. Bacteria were manually dispensed onto the medium, and the colonies of the bacteria were observed to grow for at least 24 h before the medium dried. Poly(vinyl alcohol) with polymerization initiator was also successfully applied as a medium. This bioassay system is expected to be applied for a variety of bacterial and yeast spores, and enzymes.