Dissecting divergent local circuit function during memory formation in hippocampus

正如2021财年F-7-1年终报告所报告的那样，钙记录的主要数据集的完成速度比预期的要快，从而有时间扩展数据集。这是在2022财年进行的。我用即时早期基因（记忆标记）神经元的双成像记录先导小鼠，以及所有神经元的动态活动来验证该系统，并进行了小鼠记录的额外数据集来验证先前结果的作用机制。特别的是，我直接记录了我之前的研究结果中涉及到的细胞，用荧光指示器在活的老鼠身上显示，化学药物关闭或激活这些神经元的效果确实如预期的那样有效。这个数据集也可以被分析，以进一步了解在执行的行为任务中记忆是如何增强或中断的。我进一步对之前的主要数据集进行了重要的数据分析，并开发了一种新的算法，用于使用统计度量和变分贝叶斯高斯混合模型等机器学习工具，从开放场环境中记录的神经元中检测“位置场”信息。我还完成了一个新的数据集，对向本研究涉及的海马体部分提供输入的大脑区域进行跟踪研究，以表明早期进行的操作导致了与操作模式一致的ieg介导的突触接触的变化，以验证记忆变化的长期影响。

As reported in the FY2021 F-7-1 year end report, the main dataset of calcium recordings were completed more rapidly than expected, allowing time to expand the dataset. This was preformed in FY2022. I recorded pilot mice with dual imaging of immediate early gene (memory labelling) neurons as well as dynamic activity of all neurons to verify the system, and also performed an additional dataset of recordings of mice to verify the mechanism of action of the previous results. In particular, I recorded directly from the cells implicated in my earlier results using fluorescence indicators in live behaving mice to show that the effect of the chemogenic drug to shut down or activate those neurons actually worked as expected. This dataset can also be analyzed for additional insights as to how the memory is enhanced or disrupted in the behavioral tasks performed.I furthermore performed significant data analysis of the previous main dataset and developed a novel algorithm for detecting “place field” information from the recorded neurons in an open field environment using statistical measures and machine learning tools like varational Bayesian Gaussian mixture models.I was also able to complete a new dataset on performing tracing studies on the brain regions that provide inputs to the part of hippocampus implicated in this study, to show that the manipulation performed earlier results in changes in IEG-mediated synaptic contacts consistent with the pattern of manipulation performed, to verify the long-term effects of the memory changes.