Combined investigations on the temperature and flow field on an acoustically-driven microfluidic 2D-single cell per well analysis system

该项目的目的是表征基于表面声波（SAW）的2D单细胞/孔微流体分析系统。第一次，细胞（模型颗粒）的位置，周围的流场以及温度场将分析具有高空间和时间分辨率的测量技术。以这种方式，可以研究高频声波（> 100 MHz）对细胞的影响。SAW分布的激光多普勒测振仪和速度和温度场的像散粒子跟踪的实验表征允许分析基本的流体力学和热动力学现象，以帮助可靠的细胞分析系统的设计，并建立数值模型进行模拟。最后，将推导出细胞上的机械应力和热应力，以使生物学实验能够长期进行，而不会对细胞造成损伤。然而，在该项目的过程中，重点是物理现象，将不使用活细胞，以确保可重复性，并避免使用生物样品带来的额外复杂性。

The aim of the project is the characterization of a 2D single cell per well microfluidic analysis system based on surface acoustic waves (SAW). For the first time, the position of the cell (model particle), the surrounding flow field as well as temperature field will be analysed with measurement techniques featuring high spatial and temporal resolution. In that way, the effects of high frequency acoustic waves (> 100 MHz) on the cells can be investigated. The experimental characterization of the SAW distribution by laser Doppler vibrometry and the velocity and temperature field by astigmatism particle tracking allows to analyse the underlying fluid mechanical and thermo dynamical phenomena, in order to help for the design of reliable cell analysis systems and establish numerical models for their simulation. Finally, the mechanical and thermal stress on the cells will be derived to enable long term experiments in biology without damage on the cells. However, during the course of this project the focus is on the physical phenomena and no living cells will be used, to ensure repeatability and avoid additional complexities from the use of biological samples.