Upgrade 600 MHz solid-state NMR console including MAS probe head

我们申请一个固态核磁共振光谱仪的交换控制台，包括一个超快MAS探头，促进MAS频率高达111 kHz，以解决以下研究课题：疾病相关蛋白IAPP， asas（1-42）和PrP的淀粉样原纤维的结构和功能研究。我们的目标是通过高分辨率固态核磁共振光谱来阐明这些淀粉样原纤维的三级和四级结构，研究小分子与原纤维表面的配体结合。纤维的结构重排随pH值和盐浓度的变化而变化。此外，我们将在分子水平上表征低聚亚ß（1-42）和天然折叠PrP之间的相互作用。最后，功能性淀粉样蛋白TasA的生物膜形成可以在单体、低聚体和成熟淀粉样原纤维中进行研究，将通过确定低聚体和原纤维形式的结构来详细表征。除了经典的固态核磁共振13C和15N检测实验外，我们还将应用基于1H检测的实验。为此目的，一个高速MAS探头促进旋转速度高达111千赫是强制性的。除了共振分配和结构确定的实验外，我们还将应用和改进局部动力学研究的实验。为此，膜蛋白OmpX将作为模型物质。

We apply for an exchange console for a solid-state NMR spectrometer, including an ultrafast MAS probe head facilitating MAS frequencies up to 111 kHz to address following research topics:Structural and functional investigation of amyloid fibrils of the disease relevant proteins IAPP, Aß(1-42) and PrP: We aim to elucidate tertiary and quarternary structure of these amyloid fibrils by high-resolution solid-state NMR-spectroscopy, study ligand binding of small molecules to the surface of the fibrils. Structural rearrangements of fibrils upon the change of pH and salt concentration will be determined. Further, we will characterize the interaction between oligomeric Aß(1-42) and natively folded PrP on the molecular level. Finally, the biofilm formation of the functional amyloid TasA which can be studied in monomeric, oligomeric and as mature amyloid fibrils will be characterized in detail by determining the structures of the oligomeric and the fibrillar form.Besides classical solid-state NMR experiments involving 13C and 15N detection, we will also apply experiments based on 1H detection. For this purpose, a high-speed MAS probe head facilitating spinning speeds up to 111 kHz is mandatory. Besides experiments for resonance assignment and structure determination we will also apply and improve experiments for investigation of local dynamics. For this purpose, the membrane protein OmpX will serve as a model substance.