Superresolution stimulated emission-depletion (STED) microscope

超分辨率荧光显微镜是为了研究细胞结构和动态的细胞过程低于阿贝衍射极限。因此，所要求的受激发射损耗（STED）显微镜将满足生物学研究所（Humboldt-Universität zu Berlin）几个研究小组在纳米尺度上的超分辨率光学显微镜（纳米显微镜）的需要。没有类似的仪器使用STED技术，可在Humboldt-Universität祖柏林。目前，生物学研究所（Humboldt- Universität zu Berlin）的超分辨率显微镜实验只能与外部机构合作，或使用a . Herrmann教授的“分子生物物理学”研究小组的光激活定位显微镜（PALM）。然而，PALM需要特殊的光激活荧光团和特定的实验条件，这往往需要重建现有的荧光蛋白融合结构。此外，现有的PALM显微镜被Herrmann教授的“分子生物物理学”研究小组和E. Klotzsch教授新成立的“实验生物物理学/机械生物学”工作组充分利用。重要的是，在Humboldt-Universität柏林大学没有其他的活细胞超分辨率显微镜，因此微生物生物学、感染生物学、细胞生物学、神经生物学和生物物理学等许多前沿研究问题目前只能通过外部合作来解决。这对于在Humboldt-Universität柏林大学的任何正在进行的和未来的生命科学研究中实现连续性、可持续性和效率都不是最佳的。主申请者M. Erhardt教授、C. Schmitz-Linneweber教授、共同申请者B. Beckmann博士（独立青年研究小组）、M. Brecht教授、P. Hegemann教授、A. Herrmann教授/Dr。T. Korte、E. Klotzsch教授、S. Reber博士（独立的初级研究小组）和N. Tschowri博士（Emmy Noether初级研究小组）强调了需要超越衍射极限的成像技术的各种研究问题和应用。因此，所要求的STED显微镜将满足生物研究所多个研究小组正在进行和计划中的许多研究项目的超分辨率成像需求。执行STED显微镜的能力将推动正在进行的项目对生物结构的空间理解达到一个新的水平，也将允许开发全新的研究线。丰富的项目，无论是正在运行的还是拟议的，都将确保所要求的显微镜的全部能力。因此，采购STED显微镜将有助于生物研究所继续开展有竞争力的研究，并将促进大量的地方、国家和国际合作。

Superresolution fluorescence microscopy is needed in order to investigate cellular structures and dynamic cellular processes below the Abbe diffraction limit.Accordingly, the requested stimulated emission-depletion (STED) microscope will serve the need for superresolution light microscopy on a nanometer-scale (nanoscopy) of several research groups at the Institute for Biology, Humboldt-Universität zu Berlin. No similar instrumentation using the STED technique is available at the Humboldt-Universität zu Berlin.At the moment, superresolution microscopy experiments at the Institute for Biology, Humboldt- Universität zu Berlin are only possible in collaboration with external institutions, or using a photoactivated localization microscope (PALM) of the research group ‘Molecular Biophysics’ of Prof. A. Herrmann. However, special photo-activatable fluorophores and specific experimental conditions are needed for PALM, which frequently requires re-construction of available fluorescent protein fusion constructs. In addition, the available PALM microscope is used to capacity by the research group ‘Molecular Biophysics’ of Prof. Herrmann and the newly established working group ‘Experimental Biophysics / Mechanobiology’ of junior Prof. E. Klotzsch. Importantly, no other live cell-capable superresolution microscope is available at the Humboldt-Universität zu Berlin and thus many cutting-edge research questions in microbial biology, infection biology, cell biology, neurobiology and biophysics can currently only be addressed in external collaborations. This is not optimal to achieve continuity, sustainability and efficiency in any ongoing and future research in the life sciences at the Humboldt-Universität zu Berlin. The attached research projects of the main applicants Prof. M. Erhardt and Prof. C. Schmitz-Linneweber, the co-applicants Dr. B. Beckmann (independent junior research group), Prof. M. Brecht, Prof. P. Hegemann, Prof. A. Herrmann/Dr. T. Korte, Prof. E. Klotzsch, Dr. S. Reber (independent junior research group) and Dr. N. Tschowri (Emmy Noether junior research group) highlight the wide variety of research questions and applications that require imaging techniques beyond the diffraction limit. Accordingly, the requested STED microscope will serve the need for superresolution imaging in many ongoing and planned research projects of several research groups at the Institute of Biology. The ability to perform STED microscopy will propel ongoing projects to a new level of spatial understanding of biological structures and will also allow the development of entirely new research lines. The wealth of projects, both running and proposed, will ensure operation of the requested microscope to full capacity. The procurement of a STED microscope will thus be instrumental for continued competitive research at the Institute of Biology and, not the least, will enable a large number of local, national and international collaborations.