Functional proteomics dissection of chemotactic protein diversity (A13*)

趋化蛋白的活性在多个水平上受到调节，包括翻译后修饰。在这个项目中，我们将结合蛋白质组学发现技术、系统生物学和功能验证方法来检验这一假设，即差异修饰的趋化蛋白变体(蛋白形式)产生不同的旁分泌活性。因此，我们将建立一个系统水平的趋化因子蛋白形式多样性的观点，并将产生关于旁分泌信号及其修饰如何在组织内稳态和炎症过程中影响细胞信号和趋化作用的新见解。

Chemotactic protein activity is regulated at multiple levels, including post-translational modifications. In this project, we will test the hypothesis that divergent paracrine activities arise from differentially modified chemotactic protein variants (proteoforms) by combining proteomics discovery technologies, systems biology and functional validation approaches. Thereby, we will establish a system-level view of chemokine proteoform diversity and will generate new insights about how paracrine signals and their modifications affect cell signaling and chemotaxis during tissue homeostasis and inflammation.