Ultra-high performance liquid chromatograph coupled to a time-of-flight mass spectrometer with ion mobility separation (UHPLC/IMS-QTOF-MS)

2013年盖森海姆大学成立后不久，德国科学与人文理事会就建议建立和加强研究型教授职位，同时确立独立进行博士学位的权利。因此，盖森海姆大学现在因此支持新任命的研究教授habil博士教授的申请。Ralf Schweiggert，以资助与离子迁移率分离（IMS）装置和飞行时间质谱分析仪（TOF-MS）耦合的超高效液相色谱（UHPLC）系统。目前，盖森海姆大学没有UHPLC/IMS-TOF-MS系统以及高分辨率质谱仪。所要求的系统将允许对基于植物的原料、食品、饮料和加工副产品中的植物化学物质、残留物和污染物进行技术、生理和监管相关的全面和最先进的表征。除了能够进行高分辨率质量测定以确定分子和碎片离子的和式外，所要求的系统还允许基于离子迁移率的分离，否则无法分离等压和通常共洗脱的化合物。我们希望能更深入地了解植物材料，特别是食物的组成，以及它们在收获后处理、加工和储存过程中的变化。因此，该系统不仅将有助于未来新型和传统食品的开发，优化和分析，而且还将允许针对法律相关的研究问题，例如查询食品的来源或阐明在食品生产过程中应用了哪些技术。关于所请求系统的分散管理和利用概念是有针对性的，因为运行所请求系统的分析能力- -在Geisenheim大学- -只能在申请人的工作组中获得。盖森海姆大学不需要分析核心设施。高分辨率质谱工作的外部化已经过测试，并证明不适合申请人的研究目标。所要求的系统不仅可以使申请人的工作组达到最先进的水平，而且还可以理想地满足工作组研究目标的分析需求，与工作组和盖森海姆大学建立的现有方法相辅相成。最终，该设备将在整个食物链中提供无数新的创新研究机会。

Briefly after the foundation of Geisenheim University in 2013, the German Council of Science and Humanities has recommended the formation and strengthening of research-oriented professorships as well as the meanwhile established right for independently conducting doctorates. Thus, Geisenheim University now consequently supports the application of the newly appointed research professor, Prof. Dr. habil. Ralf Schweiggert, for funding of an Ultra-High-Performance Liquid Chromatography (UHPLC) system coupled to an ion mobility separation (IMS) device and a time-of-flight mass analyzer (TOF-MS).Currently, UHPLC/IMS-TOF-MS systems as well as high resolution mass spectrometers are unavailable at Geisenheim University. The requested system will allow the comprehensive and state-of-the-art characterization of technologically-, physiologically-, and regulatorily-relevant phytochemicals, residues, and contaminants in plant-based raw materials, foods, beverages, and processing by-products. Besides enabling high resolution mass determinations to determine the sum formula of molecular and fragment ions, the requested system allows the ion mobility-based separation of otherwise unresolvable isobaric and often co-eluting compounds. We expect to gain substantially deeper insights into the composition of plant materials, particularly foods, and their changes during post-harvest treatments, processing and storage. Thereby, the system will not only contribute to the development, optimization and analyses of novel and traditional foods in the future, but will also allow targeting legally relevant research questions such as the query for the origin of a food or that for elucidating which technology has been applied during food production.A decentralized management and utilization concept regarding the requested system is targeted, since the analytical competence to run the requested system is - at Geisenheim University - only available in the applicant’s workgroup. An analytical core facility is inexistent and not desired by Geisenheim University. Externalization of high resolution MS work has been tested and proven to be unsuitable for the research goals of the applicant. The requested system would not only allow the applicant’s workgroup to step up to the state-of-the-art and beyond, but would also ideally fit the analytical needs for the workgroup's research goals, being perfectly complementary to existing methods well established at the workgroup and Geisenheim University. Ultimately, the equipment would allow numerous new and innovative research opportunities along the whole food chain.