Physicochemical Study on Intra-callular Information Processing

多头绒泡菌和白细胞一样，是一种巨大的变形虫细胞。利用ginat细胞可以测量构成细胞的成分或物质的空间和时间依赖性变化。由于白细胞体积小，这种实验不能用白细胞进行。我们测量了磷脂组成的空间变化。在细胞边界处，磷脂酰乙醇胺（PE）含量占全部磷脂的46%，磷脂酰胆碱（PC）含量占32%。其他磷脂含量为：磷脂酰肌醇（PI）， 8%；磷脂酰丝氨酸（PS）， 6%；心磷脂（CL）， 4%，磷脂酸（PA）， 4%。PE含量随位置向内而降低，PC含量随位置向内而增加。在距边界约2cm处，PE和PC的含量基本相等，分别为41%和38%。当CaCl_2作用时，细胞表现出回避反应。该化学物质的应用使磷脂的组成发生了空间和时间上的变化。这些磷脂组成变化与细胞回避反应的关系有待进一步研究。紫外线照射生物体引起回避反应，产生活性氧。活性氧的产生没有经过实验验证。建立了一种灵敏、简便的检测细胞内H_2 O_2的方法。用这种方法，我们可以首先显示出角质细胞活性氧的产生。对于血小板的输血，淋巴细胞的污染导致不利的免疫反应。虽然已知紫外线照射可有效消除免疫反应，但其机制尚不清楚。我们认为这与活性氧有关。Phoborhodopsin （PR）是嗜盐菌（halobium halobium）的负趋光受体。我们成功地从嗜盐和亲碱细菌中纯化了这种受体蛋白PR，并阐明了其详细的光化学反应。少

Physarum polycephalum is a giant amoeboid cell as is leutocyte. The use of ginat cells enables to measure spatial and time-dependent change in components or substances constituting the cell. Due to its small in size, this kind of experiment cannot performed with leutocyte. We measure the spatial variation of phospholipid composition. At the frontier of cell, the content of phosphatidylethanolamine (PE) is 46 % of all phospholipids and that of phosphatidylcholine (PC) is 32 %. The contents of other phospholipids aer : phosphatidylinotitol (PI), 8% ; phosphatidylserine (PS), 6% ; cardiolipin (CL), 4% and phosphatidic acid (PA), 4%. The content of PE decreases as the location becomes interior while that of PC increases. At the position of about 2 cm from the frontier, the contents of PE and PC are almost equal, and these are 41 % and 38 %, respectively. When CaCl_2 is applied, the cell shows avoidance reaction. The application of this chemical changes the phospholipids composition spatial … More ly and time dependently. The relationship between these changes in phospholipid composition and avoidance reaction of the cell is awaited for further investigation.2. Irradiation of ultraviolet light to living organism induces avoidance reaction and produces reactive oxygen. The production of reactive oxygen is not experimentally verified. We develop a sensitive and convenient method for detection of intracelluar H_2 O_2. With this method, we can first show the production of reactive oxygen of keratinocyte. For the blood-transfusion of platelet, contamination of lympocytes leads to unfavorable immune reaction. Although it is known that irradiation of ultraviolent light is effective to eliminate the immune reaction, the mechanism is not clear yet. We can suggest that the reactive oxygen is involved.3. Phoborhodopsin (PR) is a receptor of negative photo-taxis of Halobacterium halobium. We succeed in purifying this receptor protein, PR from halophilic and alkaliphilic bacterium and clarify the detailed photochemical reactions. Less