Induction of Heat Shock Proteins (HSPs) in the Process of Heat Tolerance

本研究的目的是鉴定热休克蛋白（HSPs）和冷休克蛋白（CSPs）在弱耐热和强耐寒鼠兔体内的诱导情况，以阐明其弱耐热性的机制，并探讨热耐受性与热休克蛋白的生理相关性。（1）热休克诱导了大鼠HSP 70和家兔HSP 68。用10% SDS-PAGE检测，在鼠兔的肝、肾、肾上腺、脾、脑、骨骼肌等组织中均未检测到热休克诱导的HSP。以往的研究表明，在高温状态下，肝、肾、肾上腺和脾等组织诱导HSP 70的能力相对较强。在进一步的研究中，用抗HSP 70抗体进行了Western blotting。热休克组大鼠肝组织中均检测到HSP 70，但所有鼠兔中仅1只阳性。这些结果表明， ...更多信息 热休克蛋白的诱导与鼠兔的热耐受性弱有关。(2)10%SDS-聚丙烯酰胺凝胶电泳显示热负荷诱导HSP 68表达。在抗HSP 70抗体的Western blotting实验中，68 kD蛋白对抗HSP 70抗体有反应，但染色斑点显示较小的M。W.尺寸这种差异可以解释为包括68 kD蛋白质在内的蛋白质的分级。(3)同样，在冷休克实验中，在大鼠、兔或鼠兔中未发现CSP的诱导。但有1只大鼠冷休克后胞浆中32 kD蛋白消失。本文报道了金花鼠在冬眠过程中和冬眠后血浆中55、27、25、20 kD四种蛋白质的消失。因此，本研究结果与先前报道的花栗鼠相似，并提示不仅冬眠而且短期冷休克也可能导致类似的结果。并从热适应的角度探讨了HSP和CSP的生理意义。少

The aim of this study is to identify the inductions of HSPs (heat shock proteins) and CSPs (cold shock proteins) in weak heat-and strong cold-tolerant pika in order to clarify the mechanism of its character of weak heat-tolerance and to examine the physiological correlation between thermotolerance and HSPs, or CSPs (1) HSP 70 in rats and HSP 68 in rabbits were induced by the heat-shock. In pika rabbits, the induction of HSP by the heat-shock was not detected by 10% SDS-PAGE not only in liver tissue but also in those tissues such as kidney, adrenal gland, spleen, brain and skeletal muscle. According to previous research, tissues of liver, kidney, adrenal gland and spleen have relatively large ability of inducing HSP 70 than other tissues in hyperthermia of rat. In further study, Western blotting method with anti-HSP 70 antibody was performed. HSP 70 was detected in liver tissue of all rats under heat shock, but only one showed positive in all pikas. These results suggest that the diffic … More ulty of induction of HSPs somewhat relate no the characteristic of weak heat-tolerance in pika rabbits. (2) Induction of HSP 68 by chronic heat load instead of heat-shock was observed on 10% SDS-PAGE. In the experiment of Western blotting method with anti-HSP 70 antibody, the 68 kD protein responded to anti-HSP 70 antibody but the spot of staining showed smaller M. W. size. This discrepancy may be explained as a gradation of proteins including 68 kD protein. (3) Contrarily in cold-shock experiments, induction of CSP was not identified in rat, rabbit or pika. But one result was found that 32 kD protein in cytosol fraction vanished in one rat after cold-shock. Four kinds of plasma proteins (55, 27, 25, 20 kD) vanished in chipmunk, a hybernator, during and after the period of hybernation has been reported in previous paper. Therefore, the present finding showed the similarity to the previous report of chipmunk and suggests that not only hybernation but also short term of cold-shock may lead to the similar result. Furthermore, physiological significance of HSP and CSP was discussed from the viewpoint of thermal acclimation. Less