Study on sweating from xenotransplanted human skin

异种移植人体皮肤出汗的研究

• 批准号: 08407003
• 负责人: KOSAKA Mitsuo
• 金额: $ 6.14万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08407003/
• 关键词: Method on research for sweating; nude-SCID Mouse; Week immune mouse; Human skin transplantation; Catecholamine; VIP; Ionto-phrosis; QSART (Quantative Sudo-Motor Axon Reflex); アセチール・コリン; アドレナリン; VIP(vaso-active intestinal polypeptide); ピロカルピン

I.The experimental results of 1996In human skin transplanted to the back of 3 strains of immuno-deficient mice the function of the eccrine sweat glands of the human transplant was tested by topical intradermal application of pilocarpine, adrenaline and atropine + pilocarpine. Sweat responses were obseved in pre-selected fields of obaervation by means of video macroscope. The iodine starch reaction served as an indicator for the appearance of sweat sport and permitted the evaluation of areas wetted by sweat in the field of observation. Among 9 animals tested, the hybrids between the CB-17-scid mouse and the BALB/cA-nu mouse (BALA/cA-nu, scid) seemded to exhibit the most consistent sweating response to local pharmacological stimulation. According to histological examination, eccrine sweat glands were preserved in human skin stansplanted into the back skin of the BALB/cA-nu, scid mouse strain. The heterologous, human skin graft provides a novel moded permitting, independent of the normal … More sweat gland innervation, the analysis of moleculare receptors of sweat gland cells by which the actions of natural transmitters and pharmacological agents are transduced.II.The experimental results of 1997Experimental series I (16 experiments on 16 subjects). Acetylcholine iontophoresed at 100mg/ml standard concentration was compared with nicotine, pilocarpine, adrenaline and with vasoactive intestinal polypeptide (VIP) at 3 different concentrations (0.06,0.36,1.10 mg/ml). Cholinergic agonists induced both direct sweating reflex (DIR) and sudomotor axon reflex (AXR) Adrenaline induced a weak DIR but no AXR.With the 2 higher VIP concentrations DIR but no AXR was produced, and skin temperatures increased slightly.Experimental seriesII (6 experiments on 6 subjects). After a first VIP iontophoresis at (0.36mg/ml) which elicited DIR but not AXR.The subjects exercised for 30 min to induce sweating. After 60 min rest, sweating had disappeard and heart rate was normal, but temperatures remained elevated (oral temperature by 0.22ﾟC,skin temperatures by 0.67ﾟC) indicating a thermoregulatory stated favoring heat dissipation. VIP iontophoresis now caused larger rises of local skin temperature, elicited weak but distinct AXR with a latency of about 2.5 min and an augmented DIR. Less

I. 1996年的实验结果将人皮肤移植到3种免疫缺陷小鼠的背部，通过皮内局部应用毛果芸香碱、肾上腺素和阿托品+毛果芸香碱来测试人移植物的外泌汗腺的功能。在预先选定的观察视野中，通过视频宏观镜观察汗液反应。碘淀粉反应作为一个指标的外观汗运动，并允许评估领域的汗水润湿的观察。在9只受试动物中，CB-17-scid小鼠和BALB/cA-nu小鼠（巴拉/cA-nu，scid）之间的杂交小鼠对局部药物刺激表现出最一致的出汗反应。经组织学观察，BALB/cA-nu，scid小鼠背部皮肤移植人皮肤后，外泌汗腺得以保存。异源的人皮肤移植物提供了一种新的模式，允许独立于正常的皮肤移植物。 ...更多信息 汗腺神经支配，分析汗腺细胞的分子受体，通过这些受体传递天然递质和药物制剂的作用。以100 mg/ml标准浓度的乙酰胆碱与尼古丁、毛果芸香碱、肾上腺素及三种不同浓度（0.06、0.36、1.10mg/ml）的血管活性肠肽（VIP）进行比较。胆碱能激动剂诱导直接出汗反射（reflection，ERF）和催汗轴突反射（sudomotor axon reflection，AXR）。肾上腺素诱导弱ERF但无AXR。在第一次VIP离子导入（0.36mg/ml）后，受试者进行30分钟的运动以诱导出汗。休息60分钟后，出汗消失，心率正常，但体温仍然升高（口腔温度升高0.22 ℃，皮肤温度升高0.67 ℃），表明体温调节状态有利于散热。VIP离子导入现在引起局部皮肤温度的较大升高，引起弱但明显的AXR，潜伏期约为2.5分钟，并且增加了潜伏期。少

项目成果

Kosaka M.: "Analysis of evaporation from clothing materials by the ventilated sweat capsule method." Eur J Appl Physiol. 76. 1-7 (1997)

Kosaka M.：“通过通风汗囊法分析服装材料的蒸发。”

Hirozi Kanbara: "Stable expression and secretion of the B-cell epitope of rodent malaria from Mycobacterium bovis BCG and induction of long-lasting humoral response in mouse" Vaccine. 14(1). 54-60 (1996)

Hirozi Kanbara：“牛分枝杆菌 BCG 稳定表达和分泌啮齿动物疟疾的 B 细胞表位，并诱导小鼠持久体液反应”疫苗。

Kosaka Mitsuo: "Profilin gene expression and regulation in a temperature-sensitive breast cancercell line:tsFT101." Pflugers Arch-Eur J Physiol. 434. 341-345 (1997)

Kosaka Mitsuo：“Profilin 基因在温度敏感乳腺癌细胞系 tsFT101 中的表达和调控。”

Kosaka Mitsuo: "Adaptations to the cold." Ed by Geiser F,Hulbert A&Nicol S C,University of New England Press,Armidale, 1-178 (1996)

小坂光男：“适应寒冷。”

Nagatake T.: "Attachment of moraxlla catarrhalis to pharyngeal epithelial cells is mediated by a glycosphingolipid receptor" FEMS Microbiology Letters. 135. 305-309 (1996)

Nagatake T.：“卡他莫拉菌与咽上皮细胞的附着是由鞘糖脂受体介导的”FEMS 微生物学快报。