Analysis of sweating of human skin transplanted on the weak immune mouse

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The heterologous, human skin graft provides a novel moded permitting, independent of the normal sweat gland innervation, the analysis of molecular receptors of sweat gland cells by which the actions of natural transmitters and pharmacological agents are transduced.(1) The experimental results of 1999 combined with these of 1998Experimental series I (16 experiments on 16 subjects). Acetylcholine iontophoresed at 100mg/ml standard concentration was compared with nicotine, pilocarpine, adrenaline and with vasoactive intestinal polypeptide (VIP) at 3 different concentrations (0.06, 0.36, 1.10 mg/ml). Cholinergic agonists induced both direct sweating reflex (DIR) and sudomotor axon reflex (AXR) Adrenaline induced a weak DIR but no AXR. With the 2 higher VIP concentrations DIR but no AXR was produced, and skin temperatures increased slightly.(2) Experimental series II (6 experiments on 6 subjects). After a first VIP iontophoresis at (0.36mg/ml) which elicited DIR but not AXR. The subjects exercised for 30 min to induce sweating. After 60 min rest , sweating had disappeared and heart rate was normal, but temperatures remained elevated (oral temperature by 0.22℃, skin temperatures by 0.67℃) indicating a thermoregulatory stated favoring heat dissipation. VIP iontophoresis now caused larger rises of local skin temperature, elicited weak but distinct AXR with a latency of about 2.5 min and an augmented DIR.(3) High-field micro imaging has advantages of higher sensitivity and higher spatial resolution, and T1- and T2- weighted images offer good contrasts which is able to use mouse as a standard animal model for physiological and pathological studies.