Study on virulence factors of Vibrio parahaemolyticus

本研究探讨了副溶血性弧菌引起胃肠炎的致病机制。1)从人分离的副溶血弧菌中发现了一种与VP-TDH相关的溶血素(VP-TRH)。2)非51霍乱弧菌、拟态弧菌和霍利氏弧菌均能产生与VP-TDH/TRH相关的毒素。对这些VP-TDH/TRH家族毒素进行了提纯和鉴定。3)用单抗和多克隆抗体分析了VP-TdH/TRH的免疫学性质。4)Vp-TdH N端60位和90位氨基酸残基对Vp-TdH的免疫学特性的重要性。表现出生物学上的。5)通过制备嵌合毒素，证明了33-100个氨基酸残基对VP-TDH热稳定性的重要性。6)这些嵌合毒素还表明第89和91位氨基酸残基对红细胞特异性的重要性。7)对VP-TDH作用方式的分析表明，VP-TDH是一种“预成型毒素”。8)对副溶血弧菌菌毛进行了纯化和鉴定。9)副溶血弧菌的血凝素可能是该菌的定植因子。

In this study, we investigated the pathogenic mechanism of gastroenteritis due to Vibrio parahaemolyticus. Following results were obtained through this experiment.1) A Vp-TDH-related hemolysin (named VP-TRH) was discovered as a virulence factor from human isolates of Kanagawa phenoffienon-negative V. parahaemolyticus2) Production of Vp-TDH/TRH-related toxins by V. choleras non-51, V. mimicus, and V. hollisae was demonstrated. These VP-TDH/TRH family toxins were purified3) and iharacterized. Gene cloning of these toxins was also performed.3) Immunological properties of VP-TDH/TRH were analysed by both monoclonal and polyclonal antibodies.4) Importance of amino acid residues at 60 and 90 position from the N terminus of VP-TDH for. expressing biological. activity was-demonstrated.5) By preparing chimeric toxins, importance of amino acid residues from 33 to 100 for the heat-stability of Vp-TDH was demonstrated.6) These chimeric toxins also suggested the importance of two amino acid residues at 89 and 91 for the erythrocyte-specificity.7) Analysis of mode of action of VP-TDH revealed that VP-TDH acts as a "preforming toxin"8) Purification and characterization of pili produced by V. parahaemolytiucs were performed.9) Haemagglutinins of V. parahaemolyticus was demonstrated to be a possible colonization factor of this organism,