Study on Non-Invasive Assessment of Multiple Organ Function with In vivo ^<31>P-MRS

利用全身核磁共振装置，研究了体内移植物的功能评价方法。首先，用^&lt；31&gt；P-MRS测定灌流猪肝中的磷化合物。在本实验中，由于灌流不足，只能观察到无机磷、PME、PDE和NADH。然后，在人工心肺机的灌流过程中，我们观察了杂种犬肝脏中的磷化合物。在本实验中，可以观察到高能磷酸盐(HEP)，这表明器官中的HEP水平受到灌流流量的影响。在第二个实验中，在体内，在指定感兴趣区后，将P-MRS应用于人的肝脏(Dresse方法)。在本实验中，观察了肝脏的特定光谱。根据这一发现，我们在肝脏手术前对患者进行了活体P-MRS试验，发现肝硬变和受癌症影响的肝脏之间存在不同的光谱。这一结果表明，体内的P-MRS使用了…利用一台实验性的核磁共振装置(6.4T)，我们获得了几个用于体内MRS评估的基本数据。在冷藏期间，我们研究了大鼠离体心的核磁共振谱随温度的变化。因此，如果对T_1时间和探针灵敏度的变化的影响进行校正，则可以将不同温度下获得的光谱相互比较。根据这一发现，我们评估了威斯康星大学的溶液在离体鼠心脏上的作用，发现它是有用的。此外，我们还研究了肝脏热缺血时磷酸盐的代谢，并比较了生化法测定的HEP和NMR测定的HEP。此外，我们还研究了异位心脏移植模型的组织血流量、电导导管压力-容量曲线和超微结构病理变化。这些数据可作为体内磁共振波谱测量的基础数据。较少

Using a whole body MRI device, we investigated functional evaluation method of a graft in vivo. First of all, phosphorus compounds in perfused pig livers were measured with ^<31>P-MRS. In this experiments, only inorganic phosphate, PME, PDE and NADH could be observed, because perfusion was not sufficient. Then, we observed phosphorus compounds during perfusion by artificial heart-lung machine in livers isolated from mongrel dogs. In this experiment, high-energy phosphates (HEP) could be observed, and it was suggested that HEP levels in organs are affected by perfusion flow.In the second experiment, in vivo ^<31>P-MRS was applied for human livers, after designation of regions of interest (DRESS method). In this experiment specific spectrum of the liver were observed. Following this finding, we tried in vivo ^<31>P-MRS in patients before liver operation and found different spectra between cirrhotic liver and cancer-affected livers. This result indicates that the in vivo ^<31>P-MRS is use … More ful method for functional evaluation of the donor liver before harvesting.Using an experimental NMR device (6.4T), we obtained several basic data for evaluation of in vivo MRS. Changes of spectrum in NMR depending on temperatures were studied in isolated rat hearts for cardiac viability during cold storage. As a result, the spectra obtained at different temperatures could be compared to each other if they are corrected for the influence from changes in T_1 time and probe sensitivity. Following this finding, we assessed the University of Wisconsin solution on isolated rat hearts and found its usefulness. Furthermore, we studied metabolism of phosphates during warm ischemia of perfused livers, and compared the HEP determined by a biochemical method (HPLC) and the HEP determined by NMR.And, we studied tissue blood flow, pressure volume curves with a conductance catheter and electromicroscopic pathologic assessment in heterotopic dog heart transplantation model. These data may applied for basic data of in vivo MRS measurements. Less