Raman Spectroscopic Study of the Effect of Surface Hydration Layers on the Recognition and Response Processes of Colloidal Particles

为了研究表面水化层对细胞系统中识别和响应过程的影响，我们检查了如下所述的三种胶体系统。(1)对聚合物凝胶中水的结构进行了拉曼光谱研究。通过对水分子的O-H伸缩谱带的分析，我们认为水分子的氢网络受到网络尺寸小于5 nm的聚合物凝胶的干扰。(2)研究了聚合脂质体体系中的缔合动力学。凝集素携带脂质体和多糖脂质体之间的缔合速率常数出乎意料地小于凝集素和具有较小分子量的糖之间的缔合速率常数。(3)利用拉曼光谱研究了不同类型的N-取代吡咯烷酮在重水中的疏水水合作用。估算了由于氮原子与重水分子间的氢键作用而引起的吡咯烷酮环的反转运动所对应的势能变化。疏水水合作用对吡咯烷酮衍生物溶剂化的重要性得到了有力的证明。

To investigate the effect of surface hydration layers on the recognition and response processes in cell systems, we examined three kinds of colloidal systems as described below.(1) Raman spectroscopic study on the structure of water in polymer gels was carried out. From the analysis of O-H stretching band of water, we concluded that the hydrogen network of water molecules is disturbed by the polymer gels whose network size is below 5 nm.(2) Kinetics of association processes in polymerized liposome system were examined. Association rate constant between lectin-carrying liposome and polysaccharide-liposome was unexpectedly smaller than that between the lectin and a saccharide with a smaller molecular weight.(3) Raman spectroscopic study on the hydrophobic hydration around various kinds of N-substituted pyrrolidinones in heavy water was carried out. The enthalpic change corresponding to the inversion motion of pyrrolidinone ring due to the hydrogen bonding between nitrogen atom and heavy water molecule was estimated. The importance of hydrophobic hydration on the solvation of pyrrolidinone derivatives was strongly suggested.