Influence of hypoxia on migration potentiality and effect of antitumor drugs in human hepatoma all lines.

为了选择更有效的低氧治疗肝细胞癌的药物，我们在缺氧和缺氧条件下检测了抗肿瘤药物对2种肝癌细胞系PLC/PRF/5和HuH-7的细胞毒性。观察到丝裂霉素C在缺氧条件下对两种肝癌细胞系具有潜在的增强细胞毒性。Carboquone单独对PLC/PRF/5在缺氧条件下表现出增强的细胞毒性。另一方面，阿霉素或顺铂对两种细胞系均无细胞毒性增强作用。因此，肿瘤细胞对细胞毒素的敏感性根据肿瘤暴露的条件而改变。因此，选择用于化疗的抗肿瘤药物不仅应取决于个别肿瘤对各种药物的敏感性，而且还应仔细考虑药物在某些条件下细胞毒性的变化。在缺氧条件下培养肝癌细胞系时，基质包被滤膜的迁移活性显著增强。此外，暴露于缺氧和抗肿瘤药物对细胞系的这种活性增强得多。这一发现表明，在应用栓塞时完全杀死癌细胞是很重要的。

In order to select more effective drugs under hypoxia for treatment of hepatocellular carcinoma, the cytotoxicity of antineoplastic agents for two hepatocellular carcinoma cell lines, PLC/PRF/5 and HuH-7, was examined under both oxygenated and hypoxic conditions. Mitomycin C was observed potentially to have enhanced cytotoxicity under hypoxic conditions for both hepatoma cell lines. Carboquone showed enhanced cytotoxicity under hypoxia for PLC/PRF/5 alone. One the other hand, there was no cytotoxic enhancement of adriamycin or cisplatin in either cell line. Thus, the sensitivity of tumor cells to the cytotoxic agents altered according to the conditions to which the tumor was exposed. The selection of the antineoplastic drugs for chemotherapy therefore should depend not only on the sensitivity of individual tumors to various drugs, but the alteration of the cytotoxicity of the drugs under certain conditions should also be carefully taken into account. When hepatoma all line was cultured in hypoxic condition, the migration activity through the filter coated with matrigel was significantly enhanced. Furthermore, this activity was much more enhanced by exposure to hypoxia plus an antineoplastic agent to the cell line. This finding suggests that it is important to kill the cancer cells completely when embolization is applied.