Agr-related changes in vascular endothelial cell functions

使用幼龄大鼠（5周龄）、成年大鼠（40-50周龄）和老年大鼠（100-110周龄）评估血管内皮细胞功能的年龄相关变化。结果与结论体外培养内皮细胞（ECs）的分离：采用外植体法从幼龄和老年大鼠胸主动脉和尾动脉中分离出内皮细胞，至少可培养到第20代。细胞增殖和迁移：研究了一种新的、简便的测定内皮细胞迁移的方法。^ 3h -胸苷在ECs中的掺入量和细胞的迁移量均随大鼠年龄的增长而下降。血小板聚集和血小板/主动脉前列腺素生成：adp诱导的血小板聚集随着大鼠年龄的增长而增加。3 .大鼠血小板和主动脉内皮细胞TXA_2水平随年龄增长而升高，血小板聚集指数（血小板TXA_2浓度/主动脉PGI_2生成）随年龄增长而升高。更多因素：随着大鼠年龄的增长，尾侧内皮细胞的内皮素-1分泌增加，而尾侧动脉或内皮细胞对乙酰胆碱的内皮依赖性松弛反应和嘌呤化合物的释放减少。随着大鼠年龄的增长，这些增加和减少会引起血液收缩压的增加。与Intermedical公司合作开发一氧化氮（NO）电极。研制了一种一氧化氮电极，利用该电极可以检测到carbachol处理主动脉释放的10^<-8>M一氧化氮的存在。采用体外移植法对大鼠血管内皮细胞的生理和病理生理功能进行测定，是研究人类衰老相关血管疾病发生机制的有效方法。本研究也证实了血管疾病的发生与内皮细胞年龄相关性功能障碍之间存在密切关系。少

Age-related changes in vascular endothelial cell functions were estimated using young (5 weeks old), adult(40-50 weeks old)and old(100-110 weeks old)rats.Results and Conclusion1. Isolation of cultured endothelial cells(ECs) : From thoracic aorate and cadual arteries of young and old rats, endothelial cells were isolated by an explant method and could be cultured at least until the 20th passage.2. Cell proliferation and migration : A new and simple method for measureing the migration of ECs was developed. Both the incorporation of ^3H-thymidine into ECs and migration of the cells decreased with rat aging.3. Platelet aggregation and platelet / aortic prostanoid production : ADP-induced platelet aggregation increased with rat aging. TXA_2 levels of platelet and aortic / tha aortic ECs increased with aging, inducing increment in an index of platelet aggregation(platelet TXA_2 concentration / aortic PGI_2 production)with rat aging.4. Production of endothelium-derived contracting and relaxin … More g factors : Endotheline-1 secretion from caudal ECs increased with rat aging, while the endothelium-dependent relaxing response to acetylcholine and release of purine compounds from the caudal artery or the ECs decreased. These increments and decrements would induce an increase in blood systolic pressure with rat aging.5. Development of nitric oxide(NO)electrode : By collaborating with Intermedical Co.Ltd., a NO electrode was developed and the presence of 10^<-8>M NO, which is released by treatment of aortae with carbachol, could be detected by using the electrode.The measurement of the physiological and pathophysiological functions of rat vascular endothelial cells which were isolated by the explant method was confirmed to be a useful methods to elucidate the mechanisms of occurrence of vascular disease associated with human aging. From the present study, close relationships were also confirmed to exist between the occurrence of vascular disease and age-related dysfunction of endothelial cells. Less