A morphological and functional study on the development and regeneration of lymphatic vessels.

淋巴管的发育和再生已经用形态学和功能方法进行了研究。为了原位鉴定淋巴毛细血管和增殖细胞，采用小鼠抗大鼠淋巴单克隆抗体（B27）和增殖细胞核抗原（PCNA）与其他标记物的双重免疫染色技术。(1)大鼠淋巴的发育；在胎儿时期，淋巴管发育不全。在出生后的半天内，像碳颗粒这样的大分子被吸收到小肠的中央乳管中。24小时后，淋巴细胞内未见碳颗粒，毛细血管网络发育后淋巴细胞开始形态发育和增殖。(2)大鼠淋巴再生；胸导管结扎或切除淋巴结在最初几天内引起严重的淋巴水肿和细胞浸润，几天后出现局部粘连。淋巴内皮细胞的增殖和新生淋巴管的形成通常发生在局部改变之后。然而，在早期病理阶段，b27阳性的未成熟间充质细胞可能在所谓的“外kulasaren Saftbahnen”（Kihara, 1951）的发展中发挥了最初的作用，以吸收组织液。(3)生长因子对淋巴内皮细胞的影响；体外研究了表皮细胞生长因子（ECGF）和碱性成纤维细胞生长因子（b-FGF）对淋巴内皮细胞的影响。与血液内皮细胞相比，这两种因素对淋巴内皮细胞的增殖和形态变化的影响都不太大。这些结果表明，淋巴管的发育和再生是随血管的变化而补充的，但淋巴管在吸收组织液方面的功能，特别是在早期阶段，可能不能反映其不成熟的外观。少

The development and regeneration of lymphatic vessels have been investigated using morphological and functional approaches. In order to identify lymphatic capillaries and proliferating cells in situ, a mouse monoclonal antibody against rat lymphatics (B27) and a technique for double immunostaining of proliferating cell nuclear antigen (PCNA) with other markers have been employed.(1) Development of rat lymphatics ; During fetal stages, lymphatics have been poorly developed. Within a half day after birth, large molecules such as carbon particles were taken up into central lacteals of the small intestine. After 24 hrs, however, no carbon particle was detected in the lymphatics and the morphological development and proliferation of lymphatics followed after the development of blood capillary networks.(2) Regeneration of rat lymphatics ; The ligation of the thoracic duct or removal of lymph nodes caused severe lymph edema and cell infiltration in the first few days, and then resulted in loc … More al adhesion after several days. The proliferation of lymphatic endothelial cells and the formation of newly formed lymphatics usually occurred after the local changes. However, B27-positive immature mesenchymal cells might play an initial role in the development of so called 'extravaskularen Saftbahnen' (Kihara, 1951) for absorption of tissue fluids during the early pathological stages.(3) Effect of growth factors on lymphatic endothelial cells ; Effects of epidermal cell growth factor (ECGF) and basic fibroblast growth factor (b-FGF) on lymphatic endothelial cells have been examined in vitro. Comparing with blood endothelial cells, the effects of both factors were not so great for the proliferation and morphological changes of lymphatic endothelial cells.These results suggest that lymphatics develop and regenerate supplementarily following the changes in blood vessels, but that the function of the lymphatics in terms of the absorption of tissue fluids, particularly at the early stages, may not reflect their immature appearance. Less