Molecular pathological study on oncogene mutation and growth activityof human thyroid tumor. With special references to tumor histogenesis and progression

应用免疫组织化学方法检测232例甲状腺病变的生长活性(GA)。分化型甲状腺癌的GA(2.00%)明显低于乳腺、肺、胃、结肠癌等其他器官的腺癌。在甲状腺癌中，未分化癌的GA最高(32.67%)，滤泡状癌的GA高于乳头状癌。广泛浸润性滤泡癌的GA显著高于微侵袭性滤泡癌。我们的发现表明，使用抗体MIB-1的免疫组化研究有助于了解甲状腺肿瘤的生长特征和生物学活性。此外，我们还检测了ras癌基因(K，H，N)的点突变。我们采用显微切割-聚合酶链式反应-单链构象多态性直接测序法对从甲状腺肿瘤中提取的DNA进行了研究，虽然我们在某些情况下观察到了ras的点突变，但这项实验仍在进行中。仅部分乳头状癌发生突变。因此，我们将调查预期突变更频繁的滤泡性肿瘤，并研究富碘日本人和低碘巴拉圭人甲状腺癌的突变情况。

Growth activity (GA) in 232 thyroid lesions was examined by immunohistochemistry using antibody MIB-1. The GA for differentiated thyroid carcinoma (2.00%) was much lower than adenocarcinoma of other organs, such as breast, lung, stomach and colon. Of the thyroid carcinomas, the highest GA was observed in undifferentiated carcinoma (32.67%) , and follicular carcinomas showed a higher GA than papillary carcinomas. Widely invasive follicular carcinomas showed a significantly higher GA than minimally invasive carcinomas. Our findings suggest than immunosistochemical investigation using the antibody MIB-1 contributes to the understanding of growth characteristics and biological activities in thyroid tumors.In addition, we examined point mutations of ras oncogene (K,H,N). We employed the Microdisection-PCR-SSCP-Direct Sequence method for investigation of DNA extracted from thyroid tumor.Although we observed the point mutation of ras in some cases, this experiment is still in progress. Only some papillary carcinomas showed mutation. Therefore, we are going to investigate follicular tumors which are expected more frequent mutation, and to study the mutation in the thyroid cancers from iodine rich japanese cases and iodine poor paraguayan cases.