Pathelogical and nualecular biological study of Hepatitis C Virus infection

用巢式聚合酶链反应和酶联免疫吸附试验检测了8只实验感染丙型肝炎病毒的黑猩猩血清中丙型肝炎病毒RNA和丙型肝炎病毒相关抗体（抗假定衣壳蛋白抗体和C-100抗体）之间的关系。三种不同的模式出现从聚合酶链反应的数据：第一组（1）是急性缓解性肝炎与HCV RNA的短暂出现（2例）。第二组（第2组）慢性肝炎持续丙型肝炎病毒RNA阳性（4例）和第三组（第3组）慢性肝炎间歇出现丙型肝炎病毒RNA（2例）。在4的8只动物中，丙型肝炎病毒RNA是第一次检测到血清接种后1周。虽然在所有感染的黑猩猩血清中检测到HCV RNA，两个是阳性的，只有对假定的衣壳蛋白的抗体，而两个是阳性的，只有C-100抗原的抗体。在8例中的4例中，针对推定衣壳蛋白的抗体出现早于针对C-100抗原的抗体，在谷氨酸丙酮酸转氨酶值上升之前或与谷氨酸丙酮酸转氨酶值上升同时检测到，并且即使在恢复后仍长时间保持阳性。接种后1年，8只动物中有6只（75%）仍为丙型肝炎病毒RNA阳性，这表明丙型肝炎病毒感染中慢性携带状态的发展风险很高。此外，血清中抗体滴度与丙型肝炎病毒感染性滴度之间似乎没有良好的相关性。

The relationship between hepatitis C virus RNA and hepatitis C virus-associated antibodies (antibody against the putative capsid protein and C-100 antibody) was determined by nested polymerase chain reaction and enzyme-linked immunosorbent assay in serial serum samples obtained from eight chimpanzees experimentally infected with hepatitis C virus. Three different patterns emerged from the polymerase chain reaction data : the first (group 1) was acute resolving hepatitis with transient appearance of HCV RNA (two cases) . The second (group 2) had chronic hepatitis with persistent hepatitis C virus RNA positivity (four cases) and the third (group 3) had chronic hepatitis with intermittent appearance of hepatitis C virus RNA (two cases) . In four of eight animals, hepatitis C virus RNA was first detectable in serum 1 wk after inoculation. Although serum HCV RNA was detected in all infected chimpanzees, two were positive only for antibody against the putative capsid protein, whereas two were positive only for antibody to C-100 antigen. In four of eight cases, antibody against the putative capsid protein appeared earlier than did antibody to C-100 antigen, was detected just before or coincident with rising glutamate pyruvate transminase values and remained positive for a long time even after recovery. Six of eight animals (75%) were still hepatitis C virus RNA positive 1 yr after inoculation, suggesting that the risk of development of the chronic carrier state is high in hepatitis C virus infection. Furthermore, there did not appear to be a good correlation between antibody titer in serum and hepatitis C virus infectivity titer.