Hypothalamic hormones and central neuronal regulation following

1)伤害性刺激后立即早期基因（IEG）和肽mrna在坐骨神经下垫或撕脱伤后，我们观察了下丘脑、脊髓和脊髓神经节中c-fos和c-jun mrna的表达。观察实验后1、8、24、48小时的变化。在所有检查的区域，c-fos和c-jun mrna在1和2小时后增加。在下丘脑，CRF、加压素和脑啡肽mrna在损伤后增加。由于c-fos和抗利尿激素（或CRF） mRNA同时存在，肽mRNA的变化伴随着eg的表达。2)伤害性刺激诱导VGF在坐骨神经撕脱伤后，我们观察到下丘脑室旁核诱导VGF（一种ngf诱导肽）的产生。由于已知VGF的诱导代表了神经元的活性，因此本研究结果提示，在伤害刺激后下丘脑室旁神经元的活性增加。3)盐负荷后的复水过程为探讨应激后的恢复过程，我们采用免疫细胞化学方法检测下丘脑肽能神经元的恢复情况。我们发现抗利尿激素和丙氨酸在补液1周后恢复正常，但肌啡在补液1周后仍保持较低水平。这些发现表明，压力引起的内分泌变化即使在1周后仍在大脑中存在。

1) Immediate early genes (IEG) and peptide mRNAs following nociceptive stimulusFollowing the injection of formaline into the lower pads or avulsion of sciatic nerve, we observed the c-fos and c-jun mRNAs in hypothalamus, spinal chord, and spinal ganglion. We observed the changes 1,8,24, and 48 hours following these experiments. In all regions examined, c-fos and c-jun mRNAs were increased after 1 and 2 hours later. In the hypothalamus, CRF,vasopressin and enkephalin mRNAs were increased following the injury. Since c-fos and vasopressin (or CRF) mRNAs were coexiswted, the change of peptide mRNA accompanies the expression of IEGs.2) Induction of VGF following the nociceptive stimulusFollowing the avulsion of sciatic nerve, we observed the induction of VGF,a NGF-inducible peptide, in the paraventricular nucleus of the hypothalamus. Since it is known that the induction of VGF represents the activity of neurons, the present result suggests the activity of hypothalamic paraventricular neurons increased after the nociceptive stimulus.3) Rehydration prrocess from salt-loadingFor examining the recovery process of stress, we examined the recovery of hypothalamic peptidergic neurons by the immunocytochemical method. We found that vasopressin and galanin returned to normal after 1 week of the rehydration, but dynorphin remained low even 1 week later. These findings suggests that the stress-induced endocrine change remains even after 1 week in the brain.