Hormone regulation of c-erB-2 oncogene expression in endometrial cancer

本研究通过体外培养的人子宫内膜癌细胞系，探讨雌二醇与c-erbB-2癌基因的关系。我们检测了两种细胞系：Ishikasa细胞系（Nishida博士：Tukuba大学）。雌激素受体（ER）和孕激素受体（PR）均阳性，而OMC-2（上田博士：大坂医学）既无ER也无PR。石川系子宫内膜癌细胞在10 μ M雌二醇浓度下，7天后的增殖比对照组更受刺激<-8>。但在OMC-2中未观察到这种增殖现象。加入10 μ M雌二醇的石川细胞株<-8>在培养14天后c-erbB-2 mRNA表达增加，而不加雌二醇的石川细胞株在培养14天后c-erbB-2 mRNA表达略有下降。OMC细胞在两种条件下均无c-erbB-2 mRNA表达。c-erbB-2 mRNA的表达在激素受体介导的子宫内膜癌细胞增殖中起重要作用。

It is known that malignant transformation is a multistep process in which damage to genes normally involved in growth regulation culminates in tumor formation.An attempt was made to elucidate the relationship between the estradiol and c-erbB-2 oncogene in the human endometrial cancer cell line in vitro. we examined two of celline ; Ishikasa cell line (Dr.Nishida : Tukuba Univ.) having estorogen reseptor positive (ER) and progesterone receptor positive (PR), and OMC-2 (Dr.Ueda : Osaka Med) having neiter ER nor PR.The proliferation of endometrial cancer cells of Ishikawa line was more stimulated after the 7 days than the control at concentraion 10 ^<-8>M estradiol. But such the proliferation phenomenon was not observed in OMC-2. Ishikawa cell line added estradiol at a concentration of 10^<-8>M was presented the c-erbB-2 mRNA expression, but Ishikawa cell line without estrdiol stimulation was slightly decreased after 14 days culture. OMC cell line was not presnted the c-erbB-2 mRNA expression in the both conditions. The c-erbB-2 mRNA expression can play an important role in the development of cell proliferation mediated throught the hormone receptor in the human endometrial cancer.