Studies on the Factors Dominating Liposome Size, Size Distribution and Permeability

磷脂囊泡或脂质体通过使用疏水多孔珠、Amberlite XAD-2或从洗涤剂-磷脂混合胶束透析除去洗涤剂来制备。发现形成的脂质体主要是单层囊泡。脂质体的大小取决于去污剂的去除方法和去污剂的类型。磷脂-洗涤剂混合物聚集体的形式随着洗涤剂含量的降低而变化，通过准弹性光散射、Sephacryl S-1000上的凝胶排阻色谱、电子显微镜和悬浮液的浊度进行了追踪。在此基础上，提出了混合胶束向脂质体转化的可能机理模型。该模型的特点是由一个链状聚集体进入脂质体形成之前的中间状态。脂质体的大小及其分布受通过瞬时状态的时间因素的支配。

Phospholipid vesicles, or liposomes were prepared by detergent removel using hydrophobic porous beads, Amberlite XAD-2, or dialysis from detergent-phospholipid mixed micelles. The liposomes formed were found to be mostly unilammellar vesicles. The liposome size was dependent on the methods of detergent removal and a type of detergents. Changes in forms of phospolipid-detergent mixture aggregates with decreasing detergent content-were pursuited by quasi elastic light scattering, gel-exclusion chromatography on Sephacryl S-1000, electron microscopy, and turbidity of the suspension. On the basis of the data, a possible model of the mechanism for transformation of the mixed micelle to liposome was proposed. The model is characterized by a chain-like aggregation entering an intermediate state before liposome formation. The liposome size and its distribution are dominated by the time factor in passing the transient state.