Kinetics of Microaqueous Bioreactor System

为了在微有机溶剂中建立酶反应的动力学，定量研究在微有机溶剂中表现出水分子的状态，以及在两种不同类型的酶反应中。最令人感兴趣的结果显示了以下的内容：1.该实验程序已被证明是在有机溶液中分离分离的蛋白质结合水和游离水。正常语言-类型的吸附剂在蛋白质绑定的水和自由水被溶解在水-有限的有机溶剂中，而在非正常的单域化isotherms在物质绑定的水和自由水被溶解在水-有限的有机溶剂中。Adsorption isotherms depend also on the purity of enzyme proteins， implying that water binds also impurities existing in the enzyme preparations. 2.脂肪由脂质在两个液体相位之间的两个批次和连续的糖基化酶 ... More E和液体脂肪相位一直在研究。减少Triglyceride含量的初始反应率增加了1.7的水分含量。免费的脂肪酸在糖基化阶段以下的4%的水分含量难以形成，但在4%以上明显地开始形成。脂脂的半衰期在40摄氏度时约21天。脂肪过氧化物并不只是抑制了糖基化反应，但也是不活跃的，但它们并不严肃。15-羟基十二烷酸在脂质体功率下悬挂的15-羟基内酯（乳酸化）反应已被研究。对湿度的影响（两种边界和自由水）的内容对初始反应率和产品的产量都很明显。反应率首先增加，然后减少，因为流动内容中的增加，在特定流动内容中增加最大比率。收益率几乎达到100%，下降的湿度含量。它被包括在较低的moisture内容中，因此反应被限制在较低的moisture内容中，而它被限制在真正的可逆反应中。Less（低）

To establish the kinetics of enzynatic reaction in microaqueous organic solvent, quantitative investigations were performed on the states of water molecules in the microaqueous organic solvent, and on two different types of enzymatic reactions. The interesting results mentioned below were obtained.1. Experimental procedure was established to determine separately protein-bound water and free water dissolved in organic solvent. Normal languimier-type ad-sorption isotherms were observed between the protein-bound water and the free water dissolved in water-miscible organic solvents, while abnormal monotonically rising isotherms were obtained between the protein-bound water and the free water dissolved in water-limmiscible organic solvents. Adsorption isotherms depend also on the purity of enzyme proteins, implying that water binds also impurities existing in the enzyme preparations.2. Both batch and continuous glycerolysis of fat by lipase between two liquid phases consisting glycerik phas … More e and liquid fat phase were studied. The initial reaction rate of decreasing triglyceride content increased with the power of 1.7 of moisture content. the free fatty acid hardly formed below 4% moisture content in the glycerol phase, but started to form significantly above 4%. Half-life of the lipase was about 21 days at 40゜c. Fat peroxide not only inhibited the glycerolysis reaction but also inactivated it, but they were not serious.3. Intramolecular esterification (lactonization) reaction of 15-hydroxypentadecanoic acid by lipase power suspended in microaqueous benzene was studied. Effects of moisture (both bound- and free -water) content on both initial reaction rate and product yield were made clear. The reaction rates increased first and decreased then with the increase in moisture content, giving rise to a maximum rate at a particular moisture content. The yield increased to almost 100% at decreasing moisture content. It was concluded that the reaction was limited by hydration of the enzyme protein at lower moisture content, while it was limited by true reversible reaction at elevated moisture content. Less