Analysis of S100beta positive T-lymphocyte

我发现人S100 β ^+ T淋巴细胞（s100 TL）属于抑制性/细胞毒性T淋巴细胞亚群，并可被伴刀豆球蛋白A刺激（Virchows Arch B，53：375，1987）。我还发现S100 TL在晚期癌症患者的外周血中的数量趋于显著减少，这表明它们在肿瘤免疫中的重要作用（Blood，70：214，1987）。我发现了一例S100 β阳性T细胞白血病/淋巴瘤，并通过对以前报道的类似病例的分析，提出了“S100 β阳性T细胞白血病/淋巴瘤”作为人类T细胞恶性肿瘤的新分类（Blood，71：1299，1988）。我发现S100^+ T白血病细胞表现出自然杀伤（NK）样非特异性细胞毒性，并表达γ/δ T细胞抗原受体（TcR）异源二聚体（已提交）。用同种异体抗原和白细胞介素-2刺激正常人外周血淋巴细胞，获得了一个正常的S100 TL克隆，命名为Yt-CL 6，该克隆具有同种异体特异性细胞毒性，并表达α/β TcR异二聚体。这些结果表明，S100 TL属于细胞毒性T淋巴细胞（CTL）亚群。我还发现，S100^+ T白血病细胞和Yt-CL 6细胞（一种正常的S100 TL克隆）在佛波醇酯（TPA）刺激下表现出树突状形态学变化。结果提示，S100 TL可能是一种特殊类型的CTL，其形态可转化为类似于小鼠表皮中Thy-1^+树突状表皮细胞的树突状。

I found that human S100beta^+ T-lymphocytes (s100TL) belong to suppressor/cytotoxic T-lymphocyte subset and can be stimulated by concanavalin A (Virchows Arch B, 53: 375, 1987). I also found that S100TL tend to markedly decresase in number in peripheral blood of patients with advanced cancer, suggesting their important role in tumor immunity (Blood, 70:214, 1987). I found a case with leukemia of S100TL and proposed "S100beta positive T-cell leukemia/lymphoma" as a new category of human T-cell malignancy through assessing similar cases reported previously (Blood, 71:1299, 1988). I found that S100^+ T-leukemia cells exhibited natural killer (NK)-like non-specific cytotoxity and expressed gamma/delta T-cell antigen receptor (TcR) heterodimer (submitted). I made a normal S100TL clone by stimulation of normal human peripheral blood lymphocytes with alloantigen and interleukin-2.The S100TL clone named Yt-CL6 exhibited allospecific cytotoxity and expressed alpha/beta TcR heterodimer. These findings indicate that S100TL belong to cytotoxic T-lymphocyte (CTL) subset. I also found that the S100^+ T-leukemia cells and Yt-CL6 cells (a normal S100TL clone) exhibited dendritic morphological changes upon stimulation with phorbol ester (TPA). The results suggest that S100TL may be a special type of CTL which can convert their morphology in dendritic shapes resembling Thy-1^+ dendritic epidermal cells in murine epidermis.