Effecls of antioxidant enzyme on blcomycin-induced lung injury.

博来霉素（BLM）诱导的肺损伤具有急性炎性和晚期纤维化的特点。特别是在急性期，活性氧的参与已被报道。为了研究抗氧化酶治疗对BLM致肺损伤发展的影响，我们在给药前气管内灌注过氧化氢酶（CAT）。将大鼠分为盐水预处理组和猫预处理组，分别于给药后第1、3、7、14天处死。通过cat滴注，在第1天观察到BALF液中cat活性增强，但在实验期间，两组肺组织中cat活性无差异。在两组中，BAL显示炎症细胞急剧增加，超过一半的细胞恢复为多核白细胞。两组间BAL液蛋白含量无显著差异。两组肺组织中脂质过氧化物含量逐渐升高，但在第14天，cat预处理组的过氧化物含量显著低于盐水预处理组。在第14天，盐水预处理组胶原蛋白中最常见的氨基酸羟脯氨酸含量显著升高，而猫预处理组升高不明显。研究期间，cat预处理组PaO2高于盐水预处理组，PaCO2低于盐水预处理组。从这些结果，我们得出结论，气管内给予CAT可以减轻blm诱导的肺损伤的严重程度。

Bleomycin(BLM)-induced lung injury is characterized by acute in flammatory and late fibrotic changes. Especially in the acute phase, involvement of active oxygen species have been reported. To study the effects of anti-oxidant enzyme treatment on the development of BLM-induced lung injury, we instilled catalase(CAT) intratracheally before intratracheal BLM administ-ration. Rats were devided into saline- and cat-pretreated groups and sacrificed serially on 1,3 7 and 14 days after BLM administration. by cat instillation, augmented CAT activity was observed in BALF fluid on day 1, however, there was no difference in CAT activities in lung tissues between two groups during experiment. In both groups, BAL revealed that inflammatory cells increased dramatically and more than a half of the cells recovered were polymophonuclear leukocytes as previously reported. BAL fluid protein contents were not different between two groups. Lipid peroxides in lung tissues increased gradually in both groups, however on day 14th, in CAT-pretreated group, lipid peroxide content was significantly less than that in saline-pretreated group. The content of hydroxyproline, a most prevalent amino acid in collagen, increased significantly on day 14th in saline-pretreated group, however in cat-pretreated group, the increase was not marked. During the study period, CAT-pretreated group showed higher PaO2 and lower PaCO2 values than saline-pretreated group did. From these results, we conclude that intratracheal CAT administration could lessen the severity of BLM-induced lung injury.