Studies on the Induction of Immune Cells and Activating Mechanisms by Substances Derived from Oxoplasma Bgondii

本研究的目的是对弓形虫溶胞抗原（TLA）和Obioactin的结构和功能进行分析，并分离和合成其功能组分。1. obioactin的功能（一）.与Obioactin共同培养的巨噬细胞内cAMP、cGMP、cGMP和pH水平无明显变化。（二）、Obioactin对脾细胞的细胞毒活性无影响。2. TLA的功能（一）.接种TLA或TLA致敏的小鼠脾细胞可抑制小鼠移植性肿瘤的增殖。（二）、Thy-1。TLA致敏小鼠脾脏中Lyt-1、Lyt-2、asialoGM-1和sIg阳性细胞及胸腺中Thy-1阳性细胞增多。（三）、当脾细胞与TLA一起培养时，诱导出细胞毒性细胞。并且，细胞毒活性可被抗去唾液酸GM 1或抗Thy-1血清阻断，但不被抗Lyt-2血清阻断。（四）、当细胞与细胞之间的相互作用 ...更多信息 Nic淋巴细胞和脾粘附细胞被阻断，没有诱导任何细胞毒性细胞。（五）、脾细胞与TLA共培养的上清无细胞毒活性和IL-2活性。（六）、TLA可刺激脾细胞的囊胚形成。3. Obioactin的纯化、合成及其功能。（一）. Obioactin的功能成分被合成为活性肽（称为生物肽）。胸腺素原-α中存在相同氨基酸序列的生物肽。（二）、寡肽抑制弓形虫在巨噬细胞、单核细胞和心肌细胞中的生长。（三）、表观肽能促进巨噬细胞释放活性氧。（四）、生物肽对小鼠移植性肿瘤的增殖有抑制作用。4. TLA活性部位的纯化、合成及合成TLA的作用。（一）.从TLA中分离得到生物活性肽，合成了TLA-H6 E。（二）、TLA-H6 E与泛素的氨基酸序列相似性在90%以上。（三）、当脾细胞与TLA-H6 E一起培养时，诱导细胞毒性细胞。少

The purposes of this research project were the analysis of function and structure of Toxoplasma lysate antigen TLA) and Obioactin, and isolation and synthesis of functional components of them. 1. The Functions of obioactin. (1). The intracellular cAMP, cGMP, cGMP and pH levels of macrophages cultured with Obioactin did not changed. (2). Cytotoxic activities of splenocytes were not influenced by Obioactin. 2. The Function of TLA. (1). An inoculation of TLA or splenocytes of mice which were sensitized with TLA inhibited the multiplication of transplanted tumor in mice. (2). The Thy-1. Lyt-1, Lyt-2-, asialoGM1 and sIg positive cells in the spleen and Thy-1 positive cells in the thymus of mice which where sensitized with TLA increased. (3). When the splenocytes were cultured with TLA, cytotoxic cells were induced. And, the cytotoxic activity was blocked by treatment with anti-asialoGM1 or anti-Thy-1 serum, but not blocked by anti-Lyt-2 serum. (4). When cell to cell interaction between sple … More nic lymphocytes and splenic adherent cells was blocked any cytotoxic cells were not induced. (5). The supernatant which splenocytes were cultured with TLA did not show cytotoxic activity and IL-2 activity. (6). The blastogenesis of splenocytes was stimulated by TLA. 3. Purification and synthesis of Obioactin, and Function of Synthesized Obioactin. (1). The functional components of Obioactin was synthesized as active peptides (named obiopeptides). There is the same amino acid sequence obiopeptides in prothymosin-alpha. (2). Obiopeptides inhibited the growth of Toxoplasma gondii in macrophages, monocytes and heart cells. (3). Obiopeptides enhanced the release of the active oxygens from macrophages. (4). The multiplication of transplanted tumor in mice was inhibited by obiopeptides. 4. Purification and synthesis of active part of TLA, and action of synthesized TLA. (1). Biological active peptide was isolated from TLA, and TLA-H6E was synthesized. (2). The amino acid sequence of TLA-H6E and ubiquitin was similar more than 90 %. (3). When the splenocytes were cultured with TLA-H6E, cytotoxic cells were induced. Less