CULTURE OF CEREBRAL MICROVASCULAR ENDOTHELIAL CELL AND APPLICATION FOR THE ANALYSIS OF THE CEREBRAL ISCHEMIA

(1)通过测定花生四烯酸钠对培养的蒙古沙鼠脑微血管内皮细胞的体外细胞毒作用，测定花生四烯酸钠对培养的蒙古沙鼠脑微血管内皮细胞的体外细胞毒作用。观察环氧合酶和脂氧合酶抑制剂（吲哚美辛和去甲二氢愈创木酸）对体外培养内皮细胞的保护作用。花生四烯酸钠在最小浓度为3mug/ml时损伤内皮细胞，其细胞毒性与花生四烯酸钠浓度和孵育时间有关。北二氢愈创木酸抑制花生四烯酸钠对内皮细胞的损伤。另一方面，吲哚美辛对内皮细胞无保护作用。推测内皮细胞损伤是由脂氧合酶产物引起的。(2)花生四烯酸酯衍生物对体外脑内皮通透性的影响将沙鼠脑内皮细胞培养于胶原包被膜滤膜上，观察花生四烯酸酯衍生物对fitc -白蛋白通过内皮细胞的影响。前列腺素E_2和F_2 α没有增加内皮细胞的通透性。15-HPETE使内皮细胞通透性有轻微升高（p< 0.05）。亮三烯C_4显著增加内皮通透性（p<0.001）。白三烯可能通过增加脑微血管内皮通透性在缺血性脑损伤的发生发展中起重要作用。

(1) Cerebral endothelial cell damage induced by sodium arachidonate in vitroCytotoxic effect of sodium arachidonate on cultured microvascular endothelial cells derived from mongolian gerbil brain was measured by specific release of ^<51>Cr. Protective effect of cyclooxygenase and lipoxygenase inhibitors (indomethacin and nordihydroguaiaretic acid respectively) on cultured endothelial cells was also examined.Sodium arachidonate injured endothelial cells at the minimal concentration of 3mug/ml, and the cytotoxicity was dependent on the concentration of sodium arachidonate and incubation time. Nordihydroguaiaretic acid inhibited the endothelial injury by sodium arachidonate. On the other hand indomethacin had no protective effect on the endothelial cells. It is posturated that the endothelial cell injury was caused by lipoxygenase product.(2) Effect of arachidonate derivatives on cerebral endothelial permeability in vitroEndothelial cells derived from songolian gerbil brain were cultured on collagen coated membrane filter, and the effect of arachidonate derivatives on passage of FITC-albumin through endothelial cells was examined.Prostaglangin E_2 and F_2 alpha did not increase the permeability of endothelial cells. 15-HPETE increased the endothelial permeability slightly (p< 0.05). Leucotriene C_4 markedly increased the endothelial permeability (p<0.001). Leucotrienes may play an important role in the development of ischemic brain injury by increasing the cerebral microvascular endothelial permeability.