5-Laser Cell Sorter

细胞分选，也称为流式细胞术，是生命科学中的关键技术。基于流式细胞术的细胞纯化允许对细胞表型的无与伦比的定义，并且对于获得高度纯化和明确定义的细胞群以用于进一步分析（包括许多现代组学技术）是必不可少的。在RWTH亚琛大学，我们在一个核心设施-流式细胞仪设施（FCF）中协调和集中流式细胞仪技术。自2017年7月启动以来，FCF的用户和研究项目不断增长，如今细胞分选能力已成为亚琛工业大学医学院FCF和研究项目的限制因素。在这里，我们要求资助一个高端细胞分选机安装在FCF。我们迫切需要一种新的细胞分选仪来提高细胞分选能力，建立对潜在感染性人类原代细胞和生物安全S2材料的常规细胞分选，并通过一种新的最先进的仪器来扩展细胞分选选项。需要细胞分选能力的研究项目包括23个由第三方资助的高知名度研究项目，包括医学系新研究联盟资助的几个项目，CRC 1382，TRR 219和CRU 344。这些项目草图和简历证明了FCF对最先进的细胞分选能力的高要求以及各自研究项目的高质量。

Cell sorting, also referred to as flow cytometry, is a key technology in life sciences. Flow cytometry-based cell purification allows for unmatched definition of cell phenotypes and is essential to obtain highly purified and well defined cell populations for further analysis including many modern –omics technologies. At RWTH Aachen University, we coordinate and centralize flow cytometry technology in a core facility, the flow cytometry facility (FCF). Since its start in July 2017, the FCF experienced a constant grow in users and research projects and today cell sorting capacity has become a limiting factor in the FCF and research projects at the Medical Faculty at RWTH Aachen University. Here we request funding of a high-end cell sorter to be installed in the FCF. A new cell sorter is urgently needed to increase cell sorting capacity, to set-up routine cell sorting of potentially infective human primary cells and biosafety S2 material and to extend cell sorting options by a new state-of-the art instrument.Research projects that require cell sorting capacity comprise a collection of 23 third party-funded high-profile research projects, including several projects funded in new research consortia at the Medical Faculty, the CRC1382, TRR 219 and CRU 344. These project sketches and cv demonstrate the high demand on state-of-the art cell sorting capacity in the FCF and the high quality of the respective research projects.