High content screening microscope with life cell imaging unit

多年来，Willbold小组一直在研究体外细胞培养中的各种问题。这些研究包括对自噬信号通路的研究，或对Willbold集团开发的用于治疗神经退行性疾病的候选药物的作用模式的阐明。由于目前可用的仪器，这些项目在实验设计方面受到限制。活细胞不能被分析超过几分钟，因为研究所的所有显微镜都没有生命细胞成像单元，该单元在5%的CO2下提供37 °C的恒温。没有这个单元，细胞因此在分析时处于压力下，这否定了所获得的结果的意义。时间相关分析，E。G.囊泡运输过程，绝对需要一个带有生命细胞成像单元的显微镜。共焦系统也是强制性的，因为它允许Z堆叠并最大限度地减少来自焦点外光的背景荧光。此外，所有应用都受益于高度自动化、高吞吐量和高速度。特别是，诊断sFIDA（基于表面的荧光强度分布分析）技术需要一个自动化的显微镜，用于高通量筛选大量的样品。现有的设备不以任何方式满足这些要求。因此，具有生命细胞成像单元的应用高通量显微镜对于Willbold集团的项目至关重要。

For many years, the Willbold group has been investigating a wide variety of questions in in vitro cell cultures. These include studies on signalling pathways in autophagy or the elucidation of the mode of action of drug candidates developed by Willbold group for the therapy of neurodegenerative diseases. With the currently available instrumentation, these projects are limited in terms of experimental design. Living cells cannot be assayed for longer than a couple of minutes, since all microscopes in the institute lack a life cell imaging unit, which provides a constant temperature of 37 °C at 5 % CO2. Without this unit, the cells are therefore under stress at the time of analysis, which negates the significance of the results obtained. Time-dependent analysis, e. g. of vesicle transport processes, absolutely requires a microscope with a life cell imaging unit. A confocal system is mandatory as well, since it allows Z-stacking and minimizes background fluorescence from out-of-focus light. Moreover, all applications benefit from a high degree of automation, high throughput and high speed. Especially, the diagnostic sFIDA (surface-based fluorescence intensity distribution analysis) technology requires an automated microscope for high throughput screening of large sample numbers. The existing devices do not meet these requirements in any way. The applied high-throughput microscope with life cell imaging unit is therefore essential for the projects of the Willbold group.