特異的抗腫瘍効果を期待した放射線療法との併用による新しい養子免疫療法の確立

本研究的目的是通过增加靶细胞上的MHC I类抗原，建立过继性免疫治疗效果。4种人鳞状细胞癌细胞株上MHC I类抗原的升高放射和/或干扰素γ (ifn - γ)对人鳞状细胞癌细胞株上MHC I类抗原的升高2 .淋巴因子活化杀伤（LAK）细胞对人鳞状细胞癌的敏感性随MHC I类抗原的升高而降低。3 .辐射和/或ifn - γ增加了小鼠鳞状细胞癌NRS1细胞株上MHC I类抗原的表达。随着MHC I类抗原在NRS1.5上表达的增加，LAK对小鼠同种（C3H/He）和异体（C57BL/6, Balb/c） LAK细胞的细胞毒敏感性降低。5 . pH3.0试剂处理后，NRS1上MHCⅰ类抗原消失。在NRS1.7上，随着MHC I类抗原的消除，LAK对小鼠同种异体（C57BL/6, Balb/c） LAK细胞的细胞毒敏感性增加。LAK细胞亚群参与LAK敏感性变化的研究本研究提示，asialo-GM1抗原阳性细胞参与了依赖于NRS1 MHC I类抗原的LAK敏感性变化。我们正在研究CD8抗原阳性细胞在LAK敏感性变化中的作用。

The purpose of this study is buildup adoptive immunotherapy effect with increase of MHC class I antigen on target cells by radiation.1.Increase of MHC class I Antigen on Four Kinds of Cell Lines of Human Squamous Cell CartinomaMHC class I antigen on cell lines of human squamous cell cartinoma increase by radiation and/or interferon-gamma (IFN-gamma).2.Changes of Lymphokine Activated Killer (LAK) Sensitivity with Increase of MHC class I Antigen on Cell Lines of Human Squamous Cell CartinomaThe sensitivity of cytotoxicity against human allogenic LAKcells is decreased with increase of MHC class I antigen.3.Increase of MHC class I Antigen on Murine Cell Line of Squamous Cell Cartinoma NRS1MHC class I antigen on NRS1 increased by radiation and/or IFN-gamma.4.Changes of LAK Sensitivity with Increase of MHC class I Antigen on NRS1The sensitivity of cytotoxicity against murine syngenic (C3H/He) and allogenic (C57BL/6, Balb/c) LAK cells is decreased with increase of MHC class I antigen on NRS1.5.Elimination of MHC class I Antigen on NRS1MHC class I antigen on NRS1 disappeared by treatment of pH3.0 reagent.6.Changes of LAK Sensitivity with elimination of MHC class I antigenThe sensitivity of cytotoxicity against murine allogenic (C57BL/6, Balb/c) LAK cells is increased with elimination of MHC class I antigen on NRS1.7.Investigation for Subpopulation in LAK Cells which take part in the Changes of LAK SensitivityIt suggest that asialo-GM1 antigen positive cells participate in the change of LAK sensitivity dependent on MHC class I antigen of NRS1 in this study.we are investigatingthe role of of CD8 antigen positive cells in the change of LAK sensitivity.