New Therapeutic omega-3 unsaturated fatty acid for nonspecific inflammatory colonic diseases.

肠道非特异性炎症性疾病--克罗恩病、溃疡性结肠炎和肠道白塞病--的病因尚不清楚。治疗这些病症的方法是完全肠外营养以及给予类固醇和沙拉唑磺胺吡啶类免疫抑制剂。然而，众所周知，长期的完全肠外营养会导致局部免疫能力下降，并伴随着肠道的废弃萎缩。本研究的目的如下：(1)(A)针对可吸收EPA的初步开发，该EPA具有优越的抗炎作用。(B)建立利用卵磷脂进行乳化的准备技术，以及(C)通过将大豆油和鱼油混合制成含有w-3脂肪的乳状液。(2)静脉给药方法的有效性检查，…更多是通过在患病动物模型上使用新开发的脂肪营养来实现的。(3)通过对动物模型给予适当的鱼油混合物，(B)通过分析血清中的脂肪酸，以及(C)通过检查组织学结果和肠粘膜的生物功能状态，证实其作为脂肪营养的作用。结果：(1)EPA(w-3不饱和脂肪酸)的稳定产生，防止氧化，经口静脉给药成为可能。(2)实验动物模型的建立：肛门注射2，4，6-三硝基苯磺酸(2，4，6-TNBS)后7~10天建立炎性结肠炎模型。(3)应用鱼油(w-3不饱和脂肪酸)进行治疗实验。实验在第1至第5组进行。通过病理组织学检查评估大肠溃疡的愈合情况后，第2组和第3组效果显著。体重、血液等指标无明显变化。

The causes of nonspecific inflammatory diseases fo the intestinal tract--Crohn's disease, ulcerative colitis, and intestinal Behcet's disease--are unknown. The remedies for these afflictions are total parenteral nutrition and the administration of steroids and salazosulfa pyridine immunosuppressants. It is known, however, that total parenteral nutrition over a long period leads to a decline in local immunocompetence, accompanied by disused atrophy of the intestinal tract. This produces expansion of an auxiliary lesion fo the inflammatory diseases and delayd healing.The purposes of the research were as follow ;(1) (a) Preparatory development targeting the creation of an absorbable EPA,which has a superior antiinflammatory effect. (b) The establishment of preparatory technology for emulsification by the use of lecithin, and (c) the creation of an emulsion that includes w-3 fat by mixing soybean oil and fish oil. (2) Examination of the effectiveness of the pervenous-administration method, … More by using the newly developed fat nutrient on diseased-animals' models. (3) Confirmation of the effect as a fat nutrient (a) by administering an appropriate mixture of fish oil to animal models, (b) by analyzing the fatty acid in the serum, and (c) by examining the histological findings and the state of the biological function of the intestinal mucous membranes.The results :(1) The stable production of EPA (w-3 unsaturated fatty acid), the prevention of oxidization, and peroral pervenous administration became possible. (2)Creation of experimental animal models : Inflammatory colitis was created seven to ten days through anal postinjection of TNBS (2,4,6-trinitrobenzen sulfonic acid). (3) Remedial experiment using fish oil (w-3 unsaturated fatty acid)The experiment was conducted on Groups 1 through 5. After assessing the healing of large-intestinal ulcer through pathologic histological examination, significant effects were observed in Groups 2 and 3. No notable changes were observed in body weight, blood tests, etc. Less