VITAMIN E REQUIREMENT OF SEAWATER FISH LARVAE

本研究旨在探讨轮虫及黄尾鱼、鳕鱼等海水鱼幼鱼对维生素E的吸收。本实验使用了游离型和醋酸型VE。首先，利用轮虫对游离型和醋酸型VE的积累进行了研究。VE的游离形式：Y_1=0.001X_1^2+0.026X_1+0.435(r^2=0.996)乙酸酯形式的VE：Y_2=0.077X_2+0.078(r^2=0.966)y_&lt；1，2&gt；=轮虫培养基中VE的含量(Mg)。这一结果表明，在添加100 mg/101的VE之前，游离态和醋酸态的累积没有差别。然而，在添加100 mg以上的VE时，游离型VE比乙酸型VE更多地被结合到轮虫中。其次，在轮虫培养基中添加dl-乙酸型、dl-型和d型VE，并用这些强化的轮虫饲养黄尾和太平洋鳕鱼幼体。轮虫很难将醋酸盐转化为游离态。鱼苗可将醋酸盐转化为游离态的VE，但其吸收率较低。然而，不同种类的VE形态对鱼苗的生长和成活率没有影响。鱼腥藻也被用作乳化材料。由此可见，鱼体中VE在鱼体内的积累量不大，这些观察结果对设计活食品乳化材料和鱼苗微饲料有一定的参考价值。

This research is conducted to investigate the absorption of vitamin E (VE) in rotifer and seawater fish larvae such as yellowtail and Pacific cod. Different kinds of VE,such as free form and acetate form were used in this experiment.At first, the accumulation of free-and acetate-form of VE was investigated using rotifer. We obtained linear curves as follows ; Free form of VE : y_1=0.001X_1^2+0.026X_1+0.435 (r^2=0.996) Acetate form of VE : y_2=0.077X_2+0.078 (r^2=0.966) y_<1,2>=VE content (mg) of rotifer, X_<1,2>=supplementation of VE (mg) to rotifer culture medium. This result showed that free-and acetate-form are no different in accumulation till 100mg/101 of VE supplementation. However, at a level of above 100mg of VE supplementation, free type of VE is more incorporated into rotifer than acetate type.Next, dl-acetate, dl-free, and d-free forms of VE were added to rotifer medium, and yellowtail and pacific cod larvae fed these enriched rotifers. It was difficult for rotifer to convert acetate form to free form. Fish larvae can convert acetate form to be a free form of VE,but its absorption was low compared with free form. However, growth and survival rate of fish larvae were not different between these kinds of VE form.Yuglena was also used for emulsified materials. It is suggested that VE in Yuglena did not accumulate in the body of the fish very much.These observations are useful when considering the design of emulsified materials for live foods and micro-diet for fish larvae.