Tenascin in cerebrospinal fluid is a useful biomarker for the diagosis of brain tumor

Tenascin是一种细胞外基质糖蛋白，据报道主要在中枢神经系统的胶质瘤组织中表达，包括细胞相关形式和排泄形式。最近，建立了一种高灵敏的夹心式酶免疫法定量测定替马菌素。本研究测定了脑脊液中Tenascin的含量。星形细胞肿瘤患者脑脊液中Tenascin升高(100 ng/ml)。在高级别星形细胞瘤(间变性星形细胞瘤和胶质母细胞瘤)中，其浓度明显升高(&gt；300 ng/ml)，在高级别星形细胞瘤的脑脊液播散性病例中，其浓度进一步升高(&gt；1000 ng/ml)。另一方面，在非星形细胞肿瘤和非肿瘤性神经系统疾病中，除肿瘤细胞脑膜扩散、感染刺激脑膜和蛛网膜下腔出血外，Tenascin浓度均低于100 ng/ml。在经过治疗的缓解期星形细胞瘤患者中，脑脊液中的Tenascin可忽略不计(100 ng/ml)。脑脊液中Tenascin的测定有助于脑肿瘤的鉴别诊断和星形细胞肿瘤的监测。

Tenascin, an extracellular matrix glycoprotein, has been reported to be expressed predominantly on glioma tissue in the central nervous system, both in a cell associated and an excreted form. Recently, a highly sensitive sandwich type enzyme immunoassay for quantitative determination of tenascin was developed. In the present study, the amount of tenascin in cerebrospinal fluid was measured. An increase of tenascin in CSF (>100 ng/ml) was found in patients with an astrocytic tumor. The concentration was significantly higher (>300ng/ml) in high grade astrocytoma (anaplastic astrocytoma and glioblastoma) and a further increase (>1000 ng/ml) was found in cases of CSF dissemination of high grade astrocytoma. On the other hand, tenascin concentrations were less than 100 ng/ml in non-astrocytic tumors and non-neoplastic neurological diseases, expect meningeal dissemination of tumor cells, meningeal stimulation by infection, and subarachnoid hemorrhage. In cases of treated astrocytoma in remission, tenascin was negligble (<100 ng/ml) in the CSF.The measurement of tenascin in CSF is useful for differential diagnosis of brain tumors and monitoring of astrocytic tumors.